1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Aminopeptidase P2 蛋白, Human (HEK293, His)

Aminopeptidase P2 蛋白, Human (HEK293, His)

目录号: HY-P7499
产品使用指南

Aminopeptidase P2 蛋白, Human (HEK293, His),一种在 HEK293 细胞中产生的重组人氨基肽酶 P2,在 C 末端有His标记。是一种氨基酰脯氨酸水解酶,它专门从含有倒数第二个脯氨酰残基的肽中去除 N-末端氨基酸。

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描述

Aminopeptidase P2 Protein, Human (HEK293, His), a recombinant human Aminopeptidase P2 produced in HEK293 cells, has a His tag at the C-terminus. Aminopeptidase P2 is an aminoacylproline hydrolase that specifically removes the N-terminal amino acid from peptides with a penultimate prolyl residue[1].

研究背景

氨肽酶 P2(XPNPEP2;APP2)是膜结合的。氨基肽酶 P2 是 TMTP1 肿瘤归巢肽的受体。APP2 的底物特异性受到更多限制;当 X 是 Pro 或 Gly 或 Y 是具有庞大侧链的氨基酸时,它无法水解 XPro 二肽,并且很难裂解 X-Pro-Y- 肽[1][2]

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

O43895 (H22-A650)

基因 ID
蛋白结构
N-term
XPNPEP2 (H22-A650)
Accession # O43895
6*His
C-term
中文名
重组人氨基肽酶 P2,His (HEK293-expressed)
同用名
rHuAminopeptidase P2, His; XPNPEP2; Aminopeptidase P2
氨基酸序列

HTKPVDLGGQDVRNCSTNPPYLPVTVVNTTMSLTALRQQMQTQNLSAYIIPGTDAHMNEYIGQHDERRAWITGFTGSAGTAVVTMKKAAVWTDSRYWTQAERQMDCNWELHKEVGTTPIVTWLLTEIPAGGRVGFDPFLLSIDTWESYDLALQGSNRQLVSITTNLVDLVWGSERPPVPNQPIYALQEAFTGSTWQEKVSGVRSQMQKHQKVPTAVLLSALEETAWLFNLRASDIPYNPFFYSYTLLTDSSIRLFANKSRFSSETLSYLNSSCTGPMCVQIEDYSQVRDSIQAYSLGDVRIWIGTSYTMYGIYEMIPKEKLVTDTYSPVMMTKAVKNSKEQALLKASHVRDAVAVIRYLVWLEKNVPKGTVDEFSGAEIVDKFRGEEQFSSGPSFETISASGLNAALAHYSPTKELNRKLSSDEMYLLDSGGQYWDGTTDITRTVHWGTPSAFQKEAYTRVLIGNIDLSRLIFPAATSGRMVEAFARRALWDAGLNYGHGTGHGIGNFLCVHEWPVGFQSNNIAMAKGMFTSIEPGYYKDGEFGIRLEDVALVVEAKTKYPGSYLTFEVVSFVPYDRNLIDVSLLSPEHLQYLNRYYQTIREKVGPELQRRQLLEEFEWLQQHTEPLAAHHHHHH

分子量

Approximately 78.0 kDa

纯度

Greater than 80% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
参考文献

Aminopeptidase P2 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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