Amphiregulin 蛋白, Human

Amphiregulin Protein, an EGFR ligand, serves as an autocrine growth factor and mitogen for various cells, including astrocytes, Schwann cells, and fibroblasts. It promotes cellular proliferation and interacts with CNIH in its immature precursor stage. Amphiregulin's EGFR activation underscores its role in essential cellular processes, emphasizing its significance as a regulator of cell growth and function in diverse cell types. Amphiregulin Protein, Human is the recombinant human-derived Amphiregulin protein, expressed by E. coli , with tag free. The total length of Amphiregulin Protein, Human is 98 a.a., with molecular weight of 14-16 kDa.

双调蛋白是 EGF 受体 (EGFR) 的配体，可作为多种靶细胞（包括星形胶质细胞、雪旺细胞和成纤维细胞）的自分泌生长因子和有丝分裂原。它的作用延伸到促进细胞增殖，并在其未成熟前体阶段与 CNIH 相互作用。双调蛋白激活 EGFR 的能力表明它参与了关键的细胞过程，并强调了它作为各种细胞类型的细胞生长和功能调节剂的重要性。

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 124.1 ng/mL, corresponding to a specific activity is 8058 U/mg.

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  Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 124.1 ng/mL，corresponding to a specific activity is 8058 U/mg.

Human

E. coli

Tag Free

P15514 (S101-K198)

374  [NCBI]

SVRVEQVVKPPQNKTESENTSDKPKRKKKGGKNGKNRRNRKKKNPCNAEFQNFCIHGECKYIEHLEAVTCKCQQEYFGERCGEKSMKTHSMIDSSLSK

14-16 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.