Angiopoietin-1 蛋白, Human (HEK293, Fc)

Angiopoietin-1 Protein, Human (HEK 293, Fc) is a recombinant Angiopoietin-1 protein with a Fc-flag. Angiopoietin-1 Protein is a secretory ligand of tyrosine kinase that is mainly expressed by vascular endothelial cells and hematopoietic cells. Angiopoietin-1 (Ang1) is a potential growth factor for therapeutic angiogenesis and vascular stabilization^[1][2].

血管生成素-1 是“内膜内皮细胞激酶”的分泌蛋白配体。血管生成素-1 主要在生长中的血管内皮细胞和造血细胞亚群中表达。Ang 1 可以通过高度组织化的血管生成和收紧内皮细胞 (EC) 连接来诱导独特的血管重塑^[1]。
Ang1 的结构由一个羧基末端纤维蛋白原样结构域组成，该结构域可以结合Ang1 在发育和病理性血管生成过程中的血管组装、成熟和稳定、冠状静脉生成和肾小球血管保护中发挥着关键作用^[2] .

Measured by the ability of the immobilized protein to support the adhesion of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 356.9 ng/ml, corresponding to a specific activity is 2801.905 units/mg.

• 
  Measured by the ability of the immobilized protein to support the adhesion of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 356.9 ng/ml, corresponding to a specific activity is 2801.905 units/mg.

Human

HEK293

N-hFc

Q15389 (D256-F498)

284  [NCBI]

DTVHNLVNLCTKEGVLLKGGKREEEKPFRDCADVYQAGFNKSGIYTIYINNMPEPKKVFCNMDVNGGGWTVIQHREDGSLDFQRGWKEYKMGFGNPSGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNEKQNYRLYLKGHTGTAGKQSSLILHGADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTTMMIRPLDF

Approximately 57.25 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Gou Young Koh, et al. Orchestral actions of angiopoietin-1 in vascular regeneration. Trends Mol Med. 2013 Jan;19(1):31-9.  [Content Brief]

  [2]. Nuri Oh, et al. A Designed Angiopoietin-1 Variant, Dimeric CMP-Ang1 Activates Tie2 and Stimulates Angiogenesis and Vascular Stabilization in N-glycan Dependent Manner.Sci Rep. 2015 Oct 19;5:15291.  [Content Brief]