1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Angiopoietins
  4. ANG-1
  5. Angiopoietin-1 蛋白, Mouse (HEK293, His)

Angiopoietin-1 蛋白, Mouse (HEK293, His)

目录号: HY-P74412
COA 产品使用指南

血管生成素-1 (ANGPT1) 是一种分泌型糖蛋白,属于血管生成素家族。它积极调节血管形成和稳定,在血管发育中发挥关键作用。ANGPT1 还影响骨骼肌细胞并协调血管对组织损伤的反应。它在各种组织中的广泛表达凸显了它在不同生理环境中的重要性。Angiopoietin-1 蛋白, Mouse (HEK293, His) 是重组的 血管生成素 1 蛋白,由 HEK293 表达,带有 N-His 标签。Angiopoietin-1 蛋白, Mouse (HEK293, His) 全长 222 个氨基酸,分子量约为 ~28 kDa。

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描述

Angiopoietin-1 (ANGPT1) is a secreted glycoprotein that belongs to the angiopoietin family. It actively regulates blood vessel formation and stabilization, playing a pivotal role in vascular development. ANGPT1 also influences skeletal myoblasts and orchestrates the vascular response to tissue injury. Its broad expression in various tissues highlights its significance in diverse physiological contexts. Angiopoietin-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Angiopoietin-1 protein, expressed by HEK293 , with N-His labeled tag. The total length of Angiopoietin-1 Protein, Mouse (HEK293, His) is 222 a.a., with molecular weight of ~28 kDa.

研究背景

血管生成素-1 (ANGPT1) 编码血管生长因子血管生成素家族中的一种分泌糖蛋白。作为血管酪氨酸激酶信号通路中的重要配体,该蛋白积极调节血管的形成和稳定,显示出其在血管发育中的关键作用。除了其血管功能外,ANGPT1还在横纹肌中发挥作用,影响骨骼肌母细胞的增殖、迁移和分化。此外,ANGPT1 在协调血管对组织损伤的反应中发挥着至关重要的作用。该基因表现出选择性剪接,导致多种转录变体。它在各种组织中广泛表达,包括心脏、肺和其他解剖部位,强调了 ANGPT1 在不同生理环境中的重要性。

种属

Mouse

表达系统

HEK293

标签

N-His

蛋白编号

NP_033770.2 (R277-F498)

基因 ID

11600  [NCBI]

蛋白结构
N-term
His
Angiopoietin-1 (R277-F498)
Accession # NP_033770.2
C-term
同用名
AGP1; AGPT; ANG-1; Angiopoietin-1; ANGPT1
分子量

Approximately 28 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Angiopoietin-1 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Angiopoietin-1 Protein, Mouse (HEK293, His)
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HY-P74412
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