1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Angiopoietins
  4. ANG-2
  5. Angiopoietin-2 蛋白, Canine (HEK293, His)

Angiopoietin-2 蛋白, Canine (HEK293, His)

目录号: HY-P75509
COA 产品使用指南

ANGPT2/Angiopoietin-2(狗)通过与 TEK/TIE2 结合来复杂地调节血管生成,竞争性地影响 ANGPT1 信号传导。它独立诱导 TEK/TIE2 磷酸化,在没有 VEGF 的情况下,可能导致内皮细胞凋亡,从而促进血管消退。Angiopoietin-2 蛋白, Canine (HEK293, His) 是重组的 犬血管生成素 2 蛋白,由 HEK293 表达,带有 N-His 标签。Angiopoietin-2 蛋白, Canine (HEK293, His) 全长 222 个氨基酸,分子量约为 ~27.8 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥350 In-stock
10 μg ¥600 In-stock
50 μg ¥1500 In-stock
100 μg ¥2600 In-stock
500 μg ¥7500 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

ANGPT2/Angiopoietin-2 (dog) complexly regulates angiogenesis by binding to TEK/TIE2, competitively affecting ANGPT1 signaling. It independently induces TEK/TIE2 phosphorylation and, in the absence of VEGF, may cause endothelial cell apoptosis, thereby promoting vascular regression. Angiopoietin-2 Protein, Canine (HEK293, His) is the recombinant canine-derived Angiopoietin-2 protein, expressed by HEK293 , with N-His labeled tag. The total length of Angiopoietin-2 Protein, Canine (HEK293, His) is 222 a.a., with molecular weight of ~27.8 kDa.

研究背景

狗血管生成素 2 蛋白 (ANGPT2) 在与 TEK/TIE2 结合时发挥多方面的作用,竞争 ANGPT1 结合位点并有效调节 ANGPT1 信号传导。值得注意的是,即使在 ANGPT1 缺失的情况下,ANGPT2 也能够诱导 TEK/TIE2 酪氨酸磷酸化。其功能延伸至血管生成调节,在缺乏 VEGF 等血管生成诱导剂的情况下,ANGPT2 介导的细胞-基质接触松弛可能导致内皮细胞凋亡,从而导致血管退化。相反,ANGPT2 与 VEGF 协同作用,可以促进内皮细胞迁移和增殖,充当允许的血管生成信号。此外,ANGPT2 还参与淋巴管生成的调节。该蛋白与 TEK/TIE2 的相互作用(与 ANGPT1 竞争相同的结合位点)以及与 ITGA5 的相互作用强调了其在狗生物学背景下的血管生成和血管调节中的综合作用。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Canine Angiopoietin-2 at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus TIE-2. The ED50 for this effect is 214.7 ng/mL.

种属

Canine

表达系统

HEK293

标签

N-His

蛋白编号

A0A8J8 (K274-F495)

基因 ID

607616  [NCBI]

蛋白结构
N-term
His
Angiopoietin-2 (K274-F495)
Accession # A0A8J8
C-term
同用名
Angiopoietin-2; ANG-2; ANGPT2
氨基酸序列

KEEQIIYRDCAEVFKSGLTTNGIYTLTFPNSTEEIKAYCDMETSGGGWTVIQRREDGSVDFQRTWKEYKVGFGNPSGEHWLGNEFVFQVTNQQPYVLKIHLKDWEGNEAYSLYEHFYLSGEELNYRIHLKGLTGTAGKISSISQPGNDFSTKDADNDKCICKCSQMLTGGWWFDACGPSNLNGMYYPQRQNTNKFNGIKWYYWKGSGYSLKGTTMMIRPADF

分子量

Approximately 29-33 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Angiopoietin-2 Protein, Canine (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Angiopoietin-2 Protein, Canine (HEK293, His)
目录号:
HY-P75509
需求量: