1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Angiopoietins
  4. ANG-2
  5. Angiopoietin-2 蛋白, Mouse (HEK293, Fc)

Angiopoietin-2 蛋白, Mouse (HEK293, Fc)

目录号: HY-P7844
COA 产品使用指南

血管生成素 2 蛋白 (ANGPT2) 与 TEK/TIE2 结合,与 ANGPT1 竞争并调节其信号传导。即使没有 ANGPT1,ANGPT2 也会诱导 TEK/TIE2 磷酸化。Angiopoietin-2 蛋白, Mouse (HEK293, Fc) 是重组的 血管生成素 2 蛋白,由 HEK293 表达,带有 C-hFc 标签。Angiopoietin-2 蛋白, Mouse (HEK293, Fc) 全长 478 个氨基酸,分子量为 90-120 kDa。

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2 μg ¥350 In-stock
10 μg ¥850 In-stock
50 μg ¥2550 In-stock
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描述

Angiopoietin 2 protein (ANGPT2) binds to TEK/TIE2, competes with ANGPT1, and modulates its signaling. Even in the absence of ANGPT1, ANGPT2 induces TEK/TIE2 phosphorylation. Angiopoietin-2 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Angiopoietin-2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Angiopoietin-2 Protein, Mouse (HEK293, Fc) is 478 a.a., with molecular weight of 90-120 kDa.

研究背景

血管生成素 2 蛋白 (ANGPT2) 与 TEK/TIE2 结合并与 ANGPT1 竞争结合位点,从而调节 ANGPT1 信号传导。即使在没有 ANGPT1 的情况下,它也能够诱导 TEK/TIE2 酪氨酸磷酸化。在缺乏 VEGF 等血管生成诱导剂的情况下,ANGPT2 会导致细胞-基质接触松动,可能导致内皮细胞凋亡和随后的血管退化。然而,当与 VEGF 结合使用时,它可以促进内皮细胞迁移和增殖,使其成为允许的血管生成信号。ANGPT2还在淋巴管生成的调节中发挥作用。此外,它与 TEK/TIE2 相互作用,竞争与 ANGPT1 相同的结合位点,并与 ITGA5 相互作用。

生物活性

Immobilized Mouse Angiopoietin-2 at 2 μg/mL (100 μL/well) can bind Biotinylated TIE-2. The ED50 for this effect is 0.8674 μg/mL, corresponding to a specific activity is 1.15×10^3 Unit/mg.

  • Immobilized Mouse Angiopoietin-2 at 2 μg/mL (100 μL/well) can bind Biotinylated TIE-2. The ED50 for this effect is 0.8674μg/mL, corresponding to a specific activity is 1.15×103Unit/mg.
种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

O35608 (Y19-F496)

基因 ID

11601  [NCBI]

蛋白结构
N-term
Angiopoietin-2 (Y19-F496)
Accession # O35608
hFc
C-term
同用名
rMuAngiopoietin-2/ANG2, Fc ; AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand
氨基酸序列

YSNFRKSVDSTGRRQYQVQNGPCSYTFLLPETDSCRSSSSPYMSNAVQRDAPLDYDDSVQRLQVLENILENNTQWLMKLENYIQDNMKKEMVEIQQNVVQNQTAVMIEIGTSLLNQTAAQTRKLTDVEAQVLNQTTRLELQLLQHSISTNKLEKQILDQTSEINKLQNKNSFLEQKVLDMEGKHSEQLQSMKEQKDELQVLVSKQSSVIDELEKKLVTATVNNSLLQKQQHDLMETVNSLLTMMSSPNSKSSVAIRKEEQTTFRDCAEIFKSGLTTSGIYTLTFPNSTEEIKAYCDMDVGGGGWTVIQHREDGSVDFQRTWKEYKEGFGSPLGEYWLGNEFVSQLTGQHRYVLKIQLKDWEGNEAHSLYDHFYLAGEESNYRIHLTGLTGTAGKISSISQPGSDFSTKDSDNDKCICKCSQMLSGGWWFDACGPSNLNGQYYPQKQNTNKFNGIKWYYWKGSGYSLKATTMMIRPADF

分子量

90-120 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Angiopoietin-2 Protein, Mouse (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Angiopoietin-2 Protein, Mouse (HEK293, Fc)
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HY-P7844
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