Animal-Free CCL2 蛋白, Pig (His)

CCL2 Protein serves as a ligand for CCR2, inducing chemotactic responses and calcium mobilization. It attracts monocytes and basophils but not neutrophils or eosinophils. Crucial in neuropathic pain and impacting NMDA-mediated synaptic transmission in dopamine neurons, CCL2 potentially engages in MAPK/ERK-dependent GRIN2B phosphorylation. Existing as a monomer or homodimer, it attaches to endothelial cells through glycosaminoglycan side chains and interacts with TNFAIP6 via its Link domain. Animal-Free CCL2 Protein, Pig (His) is the recombinant pig-derived animal-FreeCCL2 protein, expressed by E. coli, with N-His labeled tag. The total length of Animal-Free CCL2 Protein, Pig (His) is 76 a.a., with molecular weight of ~9.42 kDa.

CCL2 蛋白作为 CC 趋化因子受体 CCR2 的配体，通过结合和激活 CCR2 启动有效的趋化反应和细胞内钙动员。它对单核细胞和嗜碱性粒细胞表现出趋化活性，同时对中性粒细胞或嗜酸性粒细胞保持非活性。CCL2 在介导周围神经损伤引起的神经性疼痛中发挥着至关重要的作用，还可以增强含有多巴胺 D1 和 D2 受体的神经元中 NMDA 介导的突触传递，可能涉及 GRIN2B/NMDAR2B 的 MAPK/ERK 依赖性磷酸化。它以平衡状态下的单体或同型二聚体存在，通过蛋白聚糖的糖胺聚糖 (GAG) 侧链与内皮细胞相连，并通过其 Link 结构域与 TNFAIP6 相互作用。

Pig

E. coli

N-His

P42831 (Q24-P99)

397422  [NCBI]

QPDAINSPVTCCYTLTSKKISMQRLMSYRRVTSSKCPKEAVIFKTIAGKEICAEPKQKWVQDSISHLDKKNQTPKP

Approximately 9.42 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<0.1 EU per 1 μg of the protein by the LAL method.