1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens Animal-free Recombinant Proteins
  3. TNF Superfamily T Cell CD Proteins NK Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. TNF Superfamily Ligands CD178/FasL
  5. Fas Ligand (FasL)
  6. Animal-Free Fas Ligand 蛋白, Mouse (His)

Animal-Free Fas Ligand 蛋白, Mouse (His)

目录号: HY-P700176AF
COA 产品使用指南

Fas 配体蛋白的细胞质形式通过抑制基因转录发挥强大的作用。这种蛋白质通过抑制特定基因的表达在调节细胞过程中发挥着至关重要的作用。Animal-Free Fas Ligand 蛋白, Mouse (His) 是重组的 无动物源 Fas 配体蛋白,由 E. coli 表达,带有 N-His 标签。Animal-Free Fas Ligand 蛋白, Mouse (His) 全长 152 个氨基酸,分子量约为 ~18.14 kDa。

无动物成分 (Animal-free) 重组蛋白,即生产过程中不使用或接触任何动物来源的材料和试剂,具有高一致性和稳定性。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
2 μg ¥550 In-stock
10 μg ¥1500 In-stock
50 μg ¥4200 In-stock
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The cytoplasmic form of Fas ligand protein exerts powerful effects by inhibiting gene transcription. This protein plays a crucial role in regulating cellular processes by inhibiting the expression of specific genes. Animal-Free Fas Ligand Protein, Mouse (His) is the recombinant mouse-derived animal-FreeFas Ligand protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free Fas Ligand Protein, Mouse (His) is 152 a.a., with molecular weight of ~18.14 kDa.

研究背景

细胞质形式的 Fas 配体蛋白通过抑制基因转录发挥强大的影响。这种蛋白质通过抑制特定基因的表达在调节细胞过程中发挥着至关重要的作用。通过阻止基因转录,Fas 配体蛋白可以调节各种生物途径并影响细胞行为。了解这种蛋白质的功能机制可以提供对基因调控的见解,并有可能导致开发控制各种生物系统中基因表达的创新方法。

生物活性

Measure by its ability to induce apoptosis in Jurkat cells. The ED50 for this effect is <1 μg /mL.

种属

Mouse

表达系统

E. coli

标签

N-His

蛋白编号

Q544E9 (Q128-L279)

基因 ID

14103  [NCBI]

蛋白结构
N-term
His
Fas Ligand (Q128-L279)
Accession # Q544E9
C-term
同用名
soluble Fas Ligand (sFasL); TNFSF6; CD95L; Apo I Ligand; APTL; APT1LG1; CD178; Fas-Lg; Tnfs; Tnlg1a; gld
氨基酸序列

QIANPSTPSEKKEPRSVAHLTGNPHSRSIPLEWEDTYGTALISGVKYKKGGLVINETGLYFVYSKVYFRGQSCNNQPLNHKVYMRNSKYPEDLVLMEEKRLNYCTTGQIWAHSSYLGAVFNLTSADHLYVNISQLSLINFEESKTFFGLYKL

分子量

Approximately 18.14 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a solution containing 1X PBS, pH 8.0.

内毒素含量

<0.1 EU per 1 μg of the protein by the LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Animal-Free Fas Ligand Protein, Mouse (His)
目录号:
HY-P700176AF
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