Animal-Free FGF-9 蛋白, Human (His)

FGF-9 Protein plays a pivotal role in embryonic development, governing cell proliferation, differentiation, and migration. Its diverse functions include glial cell growth, differentiation, gliosis in brain repair, neuronal cell differentiation and survival, and promoting growth in glial tumors. Operating as a monomer or homodimer, FGF-9 engages in intricate interactions with FGFRs, forming critical molecular alliances for diverse cellular responses. The potentiated binding affinity with heparan sulfate glycosaminoglycans highlights FGF-9's indispensable role in orchestrating crucial cellular events. Animal-Free FGF-9 Protein, Human (His) is the recombinant human-derived animal-FreeFGF-9 protein, expressed by E. coli, with C-His labeled tag. The total length of Animal-Free FGF-9 Protein, Human (His) is 208 a.a., with molecular weight of ~22.14 kDa.

FGF-9 蛋白在调控胚胎发育、细胞增殖、分化和迁移中发挥着关键作用。它涉及多种过程，包括发育过程中的神经胶质细胞生长和分化、损伤后大脑修复和再生中的神经胶质增生、神经元细胞的分化和存活以及神经胶质瘤生长的促进。FGF-9 作为单体或同二聚体发挥作用，与 FGFR1、FGFR2、FGFR3 和 FGFR4 进行复杂的相互作用，形成对多种细胞反应至关重要的分子联盟。FGF-9 与其受体之间的结合亲和力由充当重要辅助受体的硫酸乙酰肝素糖胺聚糖增强。这些相互作用强调了 FGF-9 在协调各种生理环境中的关键细胞事件中的多方面且不可或缺的作用。

Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <2 ng/mL.

Human

E. coli

C-His

P31371 (M1-S208)

2254  [NCBI]

MPLGEVGNYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS

Approximately 22.14 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.