1. 重组蛋白
  2. Cytokines and Growth Factors Animal-free Recombinant Proteins
  3. FGF Family
  4. Fibroblast Growth Factor
  5. FGF-2/bFGF
  6. Animal-Free FGF basic/bFGF 蛋白, Human (154a.a, His)

Animal-Free FGF basic/bFGF 蛋白, Human (154a.a, His)

目录号: HY-P7331AF
COA 产品使用指南

FGF-2/bFGF 是成纤维细胞因子家族的成员,参与骨的愈合、软骨修复、骨修复和神经再生。FGF-2 也是一种有丝分裂促进剂,能加速细胞增殖。FGF-2 通过特异性靶向酪氨酸激酶受体,激活 FGF/FGFR 信号通路来调控免疫过程。例如,FGF-2 参与 JAK-STAT 信号通路来调控软骨代谢,也激活 ERK 信号促进软骨再生。FGF-2 结合 FGFR1/3 分别促进关节软骨的退化和修复。FGF-2 还是 GBM 中已知的致癌因子,有助于胶质瘤生长、血管化。无动物源,重组人碱性 FGF-2/bFGF 蛋白 (154a.a, His),是由 E.coli 大肠杆菌产生的重组蛋白,全长 154 个氨基酸,带有 N 端 His 标签。

无动物成分 (Animal-free) 重组蛋白,即生产过程中不使用或接触任何动物来源的材料和试剂,具有高一致性和稳定性。

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描述

FGF-2 is a member of the fibroblast family involved in bone healing, cartilage repair, bone repair, and nerve regeneration. FGF-2 is also a mitotic promoter that accelerates cell proliferation. FGF-2 regulates immune processes by specifically targeting tyrosine kinase receptors and activating the FGF/FGFR signaling pathway. For example, FGF-2 is involved in the JAK-STAT signaling pathway to regulate cartilage metabolism and also activates ERK signaling to promote cartilage regeneration. FGF-2 combined with FGFR1/3 promoted degeneration and repair of articular cartilage, respectively[1]. FGF-2 is also a known carcinogen in GBM, which contributes to glioma growth and vascularization[2].Animal-Free FGF basic/bFGF Protein, Human (154a.a, His), consists of 154 amino acids, produced by E.coli with tag free.

研究背景

FGF-2/bFGF 是成纤维细胞家族的成员,与肝素具有高亲和力。FGF-2在肌腱至骨愈合、软骨修复、骨修复和神经再生中发挥重要作用。FGF-2 特异性结合酪氨酸激酶受体并激活 FGF/FGFR 信号通路。随后,FGF-2通过转导其他经典途径影响细胞增殖、分化和凋亡以及免疫调节。例如,FGF-2通过调节JAK-STAT信号通路来调节软骨代谢。FGF-2 还充当有丝分裂启动子,加速细胞增殖。因此,(1)FGF-2是韧带/肌腱损伤愈合过程中重要的生长因子。体外实验显示,低剂量FGF-2可刺激骨髓间充质干细胞的增殖和分化,并上调I/III型胶原和纤连蛋白的mRNA表达。然而,高剂量的 FGF-2 不会刺激细胞外基质 (ECM) 蛋白增殖和基因表达。(2)FGF-2也是软骨修复中的内源性和内在生长因子。FGF-2 与硫酸乙酰肝素蛋白多糖结合并储存在关节软骨的 ECM 中。当软骨受损或退化时,ECM迅速释放FGF-2并激活ERK信号通路,促进软骨再生。FGF-2与其特定受体结合表现出双相效应。FGF-2与FGFR3联合促进关节软骨的修复。FGF-2 与 FGFR1 联合促进关节软骨退化[1]。FGF-2 在颗粒细胞和丘细胞以及肝细胞癌细胞中表达,但在非癌性肝组织中不表达。这揭示了 FGF-2 在脑肿瘤,特别是胶质母细胞瘤中的作用。据研究,FGF-2是GBM中已知的致癌因素。FGF-2 增强胶质母细胞瘤干细胞的自我更新,有助于胶质瘤的生长和血管化[2]。FGF-2蛋白在某些物种中高度保守,人FGF-2蛋白序列与大鼠、小鼠和牛的相似性分别为97.4%、95.45%和98.71%。

体外研究
(In Vitro)

FGF-2 (重组人源蛋白; 3 ng/mL, 30 ng/mL; 7-28 d) 在 3 ng/mL 时触发双相骨髓基质细胞 (BMSC) 反应,在第 7 天促进细胞增殖达到峰值,在第 14 和 28 天,显著增强 I 型胶原、III 型胶原、纤维连接蛋白和 α-平滑肌肌动蛋白 mRNA 表达。但在 30 ng/mL 时无明显作用[3]

生物活性

Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is <1 ng/mL. The specific activity of recombinant human FGF-2 is approximately >5 x 105IU/mg.

种属

Human

表达系统

E. coli

标签

N-His;N-His

蛋白编号

P09038-4 (A135-S288)

基因 ID
蛋白结构
N-term
His
bFGF (A135-S288)
Accession # P09038-4
C-term
中文名
无动物源,重组人碱性 FGF-2/bFGF 蛋白 (154a.a, His)
同用名
bFGF, 154a.a.; bFGF; FGF-2; HBGF-2; FGFB; Prostatropin
氨基酸序列

AAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS

分子量

Approximately 18.1 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a solution containing 0.01% sarkosyl in 1X PBS, pH 8.0.

内毒素含量

<0.1 EU per 1 μg of the protein by the LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
参考文献

Animal-Free FGF basic/bFGF Protein, Human (154a.a, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Animal-Free FGF basic/bFGF Protein, Human (154a.a, His)
目录号:
HY-P7331AF
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