Animal-Free IFN-beta 蛋白, Human (His)

PDGF R beta protein is a receptor protein that binds to platelet-derived growth factor (PDGF). It is expressed in a variety of cells, including fibroblasts and smooth muscle cells. Animal-Free IFN-beta Protein, Human (His) is the recombinant human-derived animal-FreeIFN-beta protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IFN-beta Protein, Human (His) is 166 a.a., with molecular weight of ~20.84 kDa.

IFN-β 蛋白编码属于干扰素家族的细胞因子，作为针对病原体的先天免疫反应的一部分而释放。这种蛋白质属于 I 类干扰素，对于防御病毒感染以及参与细胞分化和抗肿瘤防御至关重要。在响应病原体而分泌后，I 型干扰素结合同源受体复合物，触发炎症细胞因子和趋化因子相关基因的转录。I 型干扰素分泌的异常激活与自身免疫性疾病有关。缺乏该基因的小鼠表现出多种表型，包括 B 细胞成熟缺陷和对病毒感染的易感性增加，这强调了 IFN-β 蛋白在免疫反应和宿主防御中的关键作用。

1.Measure by its ability to induce apoptosis in HeLa cells. The ED₅₀ for this effect is <15 ng/mL.
2.Measure by its ability to induce cytotoxicity in TF-1 cells. The ED₅₀ for this effect is <0.1 ng/mL. The specific activity of recombinant human IFN beta 1a is approximately >1 x10⁷ IU/ mg.

Human

E. coli

C-His

NP_002167.1 (M22-N187)

3456  [NCBI]

MSYNLLGFLQRSSNFQCQKLLWQLNGRLEYCLKDRMNFDIPEEIKQLQQFQKEDAALTIYEMLQNIFAIFRQDSSSTGWNETIVENLLANVYHQINHLKTVLEEKLEKEDFTRGKLMSSLHLKRYYGRILHYLKAKEYSHCAWTIVRVEILRNFYFINRLTGYLRN

Approximately 20.84 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.