Animal-Free IGF-II 蛋白, Mouse (His)

IGF-II Protein plays a crucial role in glucose-mediated insulin secretion, acting as a physiological amplifier alongside insulin. Additionally, it exhibits osteogenic properties by enhancing osteoblast mitogenic activity through the phosphoactivation of MAPK1 and MAPK3. This dual functionality underscores the protein's importance in glucose homeostasis and bone metabolism, suggesting therapeutic relevance for insulin secretion and bone health contexts. Animal-Free IGF-II Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIGF-II protein, expressed by E. coli, with N-His labeled tag. The total length of Animal-Free IGF-II Protein, Mouse (His) is 67 a.a., with molecular weight of ~8.20 kDa.

IGF-II 蛋白是葡萄糖介导的胰岛素分泌的关键因素，与胰岛素共同分泌，并充当该过程的生理放大器。值得注意的是，IGF-II 通过磷酸化 MAPK1 和 MAPK3 增强成骨细胞有丝分裂活性，从而表现出成骨特性。这种双重作用强调了该蛋白质在葡萄糖稳态和骨代谢中的重要性，强调了其在与胰岛素分泌和骨健康相关的背景下的潜在治疗意义。

Measure by its ability to induce MCF-7 cells proliferation. The ED₅₀ for this effect is <6 ng/mL. The specific activity of recombinant mouse IGF-II is >1.5 x 10⁵ IU/mg.

Mouse

E. coli

N-His

D3Z4N4 (A25-E91)

16002

AYGPGETLCGGELVDTLQFVCSDRGFYFSRPSSRANRRSRGIVEECCFRSCDLALLETYCATPAKSE

Approximately 8.20 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.