Animal-Free IL-1 beta 蛋白, Pig (His)

IL-1 beta Protein, a potent pro-inflammatory cytokine, initiates various immune responses, including prostaglandin synthesis, neutrophil recruitment, T-cell cytokine production, B-cell activation, fibroblast proliferation, and collagen production. It promotes Th17 differentiation, synergizes with IL12 for IFNG synthesis by Th1 cells, and induces angiogenesis along with TNF and IL6. Involved in pyroptosis, its mature form is released through the GSDMD pore. Additionally, IL-1 beta Protein interacts with ASFV protein L83L during microbial infections. Animal-Free IL-1 beta Protein, Pig (His) is the recombinant pig-derived animal-FreeIL-1 beta protein, expressed by E. coli, with C-His labeled tag. The total length of Animal-Free IL-1 beta Protein, Pig (His) is 153 a.a., with molecular weight of ~18.51 kDa.

IL-1 β 蛋白是一种有效的促炎细胞因子，最初被发现是一种主要的内源性热原。它触发各种免疫反应，包括前列腺素的合成、中性粒细胞的募集和激活、T细胞激活和产生细胞因子、B细胞激活导致抗体产生，以及刺激成纤维细胞增殖和胶原蛋白产生。此外，它还具有促进 T 细胞 Th17 分化的作用，并与 IL12 协同诱导 Th1 细胞合成 IFNG。IL-1 β 蛋白还通过协同诱导 VEGF 与 TNF 和 IL6 的产生来促进血管生成。此外，它还参与细胞焦亡下游炎症的转导，其成熟形式通过gasdermin-D (GSDMD) 孔特异性释放到细胞外空间。在微生物感染期间，IL-1 β 蛋白还会与非洲猪瘟病毒 (ASFV) 蛋白 L83L 相互作用。

Measure by its ability to induce D10.G4.1 cells proliferation. The ED₅₀for this effect is <3 ng/mL.

Pig

E. coli

C-His

P26889 (A115-P267)

397122  [NCBI]

MANVQSMECKLQDKDHKSLVLAGPHMLKALHLLTGDLKREVVFCMSFVQGDDSNNKIPVTLGIKGKNLYLSCVMKDNTPTLQLEDIDPKRYPKRDMEKRFVFYKTEIKNRVEFESALYPNWYISTSQAEQKPVFLGNSKGRQDITDFTMEVLSP

Approximately 18.51 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.