Animal-Free IL-13 蛋白, Human (His)

IL-13 Protein is a cytokine which is secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes. IL-13 affects the morphology, growth, and surface antigen expression and phenotype of monocytes and elicits B cell proliferation. Animal-Free IL-13 Protein, Human (His) is the recombinant human-derived animal-FreeIL-13 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-13 Protein, Human (His) is 112 a.a., with molecular weight of ~13.28 kDa.

白细胞介素-13 (IL-13) 是一种细胞因子，由 2 型辅助 T (Th2) 细胞、CD4 细胞、自然杀伤 T 细胞、肥大细胞、嗜碱性粒细胞、嗜酸性粒细胞和核细胞分泌。IL-13 是 IgE 合成、杯状细胞增生、粘液分泌过多、气道高反应性、纤维化和几丁质酶上调的核心调节因子。圆二色光谱证实 IL-13 属于细胞因子的 α 螺旋家族。IL-13 与 IL2 协同调节干扰素-γ 合成。IL-13 通过 IL4R 链和 IL13RA1 链发挥生物学作用，激活 JAK1、TYK2 和 STAT6。Interleukin-13 影响单核细胞的形态、生长、表面抗原表达和表型，刺激B细胞增殖，并激活嗜酸性粒细胞、嗜碱性粒细胞和肥大细胞。在人类巨噬细胞和单核细胞中，hIL-13 已被证明可以抑制 HIV 复制。Human IL-13 还可抑制 LPS 暴露诱导的促炎细胞因子，表明作为抗炎剂的潜在治疗应用^{[1][2][3][4][5][6]}。

Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is<0.8 ng/mL. The specific activity of recombinant human IL- 13 is approximately >1 x10⁶ IU/ mg

Human

E. coli

C-His

P35225 (G35-N146)

3596  [NCBI]

MGPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKKLFREGRFN

Approximately 13.28 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.