Animal-Free IL-15 蛋白, Human (His)

IL-15 protein regulates immune cells, stimulates the proliferation of NK cells, T cells, and B cells, and promotes cytokine secretion. Animal-Free IL-15 Protein, Human (His) is the recombinant human-derived animal-FreeIL-15 protein, expressed by E. coli , with N-His, N-His labeled tag. The total length of Animal-Free IL-15 Protein, Human (His) is 114 a.a., with molecular weight of ~13.7 kDa.

IL-15 蛋白在协调针对微生物入侵者和寄生虫的炎症和保护性免疫反应、调节先天性和适应性免疫系统中的免疫细胞方面发挥着关键作用。这种细胞因子刺激自然杀伤细胞、T 细胞和 B 细胞的增殖，同时还促进各种细胞因子的分泌。值得注意的是，与大多数细胞因子不同，IL-15 在产生 IL-15 的细胞表面表达，与其高亲和力受体 IL15RA 相关，向表达 IL2RB 和 IL2RG 受体亚基的靶细胞传递信号。与其受体结合后，IL-15 会触发 JAK1 和 JAK3 的磷酸化，招募并随后磷酸化转录 3/STAT3 和 STAT5 的信号转导器和激活剂。在单核细胞中，IL-15 诱导产生 IL8 和单核细胞趋化蛋白 1/CCL2，将中性粒细胞和单核细胞吸引至感染部位。此外，在肥大细胞中，IL-15 诱导 STAT6 快速酪氨酸磷酸化，从而控制肥大细胞的存活和 IL4 等细胞因子的释放。

1.Measure by its ability to induce MO7e human megakaryocytic leukemic proliferation. The ED₅₀ for this effect is 0.5-3 ng/mL. The specific activity of recombinant human IL15 is approximately 1.5 x 10⁸ IU/mg.
2. Measure by its ability to induce NK cells proliferation. The ED₅₀ for this effect is 5-35 ng/mL.

Human

E. coli

N-His;N-His

P40933 (N49-S162)

3600  [NCBI]

NWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTSLE

Approximately 13.7 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.