Animal-Free IL-1F10/IL-38 蛋白, Human (His)

IL-1F10/IL-38 Protein is a member of the interleukin 1 cytokine family that regulates adapted and innate immune responses^[1]. Animal-Free IL-1F10/IL-38 Protein, Human (His) is the recombinant human-derived animal-FreeIL-1F10/IL-38 protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.

L-1F10/IL-38 蛋白是属于 IL-1 家族的分泌细胞因子，具有免疫调节活性。IL-1F10/IL-38 蛋白单独不会诱导细胞因子的产生，但会减少 T 细胞响应热灭活白色念珠菌而产生的 IL22 和 IL17A。IL-1F10/IL-38 蛋白可减少 IL36G 诱导的外周血单核细胞产生 IL8。IL-1F10/IL-38 蛋白可增加细菌脂多糖 (LPS) 刺激的树突状细胞产生 IL6。IL-36R/IL1RL2 的配体^[3][4]。

Measured by its ability to inhibit IL-6 secretion by THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 278.5 ng/mL, corresponding to a specific activity is 3590.664 U/mg.

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  Measured by its ability to inhibit IL-6 secretion by THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 278.5 ng/mL, corresponding to a specific activity is 3590.664 U/mg.

Human

E. coli

C-6*His

AAI03967.1 (M1-W152)

84639  [NCBI]

Full Length

MCSLPMARYYIIKYADQKALYTRDGQLLVGDPVADNCCAEKICTLPNRGLDRTKVPIFLGIQGGSRCLACVETEEGPSLQLEDVNIEELYKGGEEATRFTFFQSSSGSAFRLEAAAWPGWFLCGPAEPQQPVQLTKESEPSARTKFYFEQSW

Approximately 21 kDa, based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is recommended to reconstitute to a concentration of 100-200 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Pooria Fazeli, et al. An overview of the biological and multifunctional roles of IL-38 in different infectious diseases and COVID-19. Immunol Res. 2022; 70(3): 316–324.

  [2]. John E Sims, et al. The IL-1 family: regulators of immunity. Nat Rev Immunol. 2010 Feb;10(2):89-102.  [Content Brief]

  [3]. Frank L van de Veerdonk, et al. IL-38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist. Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):3001-5.  [Content Brief]

  [4]. Alejandro Diaz-Barreiro, et al. Multifaceted roles of IL-38 in inflammation and cancer. Cytokine. 2022 Mar:151:155808.  [Content Brief]