Animal-Free IL-25/IL-17E 蛋白, Mouse (His)

Interleukin-25 (IL-25), also known as IL-17E, belongs to the IL-17 cytokine family. IL-25 activates NF-κB, MAPKs and JAK/STAT signaling pathways^[1]. IL-25 exerts singal transduction through receptors composed of IL17RA and IL17RB^[7]. Animal-Free IL-25/IL-17E Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-25/IL-17E protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-25/IL-17E Protein, Mouse (His) is 153 a.a., with molecular weight of ~18.41 kDa.

Interleukin-25 (IL-25)，也被称为 IL-17E，属于 IL-17 细胞因子家族。IL-25 与 IL-17A 只有 16% 的同源性，其生物学效应与家族其他成员有很大不同。IL-25 最初被发现于 Th2 细胞中，也由各种免疫细胞产生，如 CD8+T 细胞、肥大细胞、巨噬细胞、树突状细胞 (DCs)、嗜酸性粒细胞、嗜碱性粒细胞、2 类先天淋巴样细胞 (ILC2s)、上皮细胞和内皮细胞^{[1][2][3][4][5][6]}。IL-25 与 IL-17RB 结合，然后与 IL-17RA 结合发挥其活性^[7]。IL-25 诱导 IL-4、IL-5、IL-13、TGF-β 和 G-CSF 的表达^[8][9]。IL-25 在非免疫细胞中可刺激细胞增殖、抑制细胞凋亡、产生炎性细胞因子和趋化因子、调节细胞-细胞粘附和细胞运动^{[10][11][12][13]}。IL-25 激活 NF-κB、MAPKs 和 JAK/STATs 信号通路^[1][14]。人和小鼠 、小鼠和大鼠的 IL-25 序列同源性分别为 78.88% 和 91.12%。

Measured by its ability to induce CXCL1 secretion in HT- 29 cells. The ED₅₀ for this effect is ≤1 ng/mL.

Mouse

E. coli

C-His

Q8VHH8 (V17-A169)

140806  [NCBI]

MVSLRIQEGCSHLPSCCPSKEQEPPEEWLKWSSASVSPPEPLSHTHHAESCRASKDGPLNSRAISPWSYELDRDLNRVPQDLYHARCLCPHCVSLQTGSHMDPLGNSVPLYHNQTVFYRRPCHGEEGTHRRYCLERRLYRVSLACVCVRPRVMA

Approximately 18.41 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.