Animal-Free IL-36 beta/IL-1F8 蛋白, Human (His)

IL-36 beta/IL-1F8 Protein, signaling through IL-36R, activates NF-kappa-B and MAPK pathways, inducing a pro-inflammatory response. Present in epithelial barriers, it shares the IL-1 system coreceptor IL1RAP. Stimulates IL-6, IL-8, antimicrobial peptides, and matrix metalloproteases. Involved in skin inflammation, influencing keratinocytes, dendritic cells, and T-cells. Induces chemokines and cytokines, promoting tissue infiltration and cell maturation. Interacts with TMED10 for translocation from cytoplasm to ERGIC for secretion. Animal-Free IL-36 beta/IL-1F8 Protein, Human (His) is the recombinant human-derived animal-FreeIL-36 beta/IL-1F8 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-36 beta/IL-1F8 Protein, Human (His) is 153 a.a., with molecular weight of ~18.17 kDa.

IL-36 beta/IL-1F8 蛋白是一种细胞因子，可结合并激活 IL1RL2/IL-36R 受体，导致靶细胞中 NF-kappa-B 和 MAPK 信号通路的激活，从而产生促炎反应。它是 IL-36 信号系统的一部分，被认为存在于上皮屏障中并参与局部炎症反应，类似于 IL-1 系统。 IL-36 beta 可刺激多种细胞类型（包括滑膜成纤维细胞、关节软骨细胞和成熟脂肪细胞）中白细胞介素 6 和白细胞介素 8 的产生。它还诱导抗菌肽和基质金属蛋白酶的表达。在皮肤中，IL-36 β 作用于角质形成细胞、树突状细胞，并间接作用于 T 细胞，促进组织浸润、细胞成熟和细胞增殖。在培养的角质形成细胞中，IL-36β诱导趋化因子和促炎细胞因子的表达，例如CCL3、CCL4、CCL5、CCL2、CCL17、CCL22、CL20、CCL5、CCL2、CCL17、CCL22、CXCL8、CCL20、CXCL1、TNF -α、IL-8和IL-6。它与货物受体 TMED10 相互作用，促进其从细胞质易位至 ERGIC 并随后分泌。

Measure by its ability to induce IL-8 secretion in human PBMCs. The ED₅₀ for this effect is <0.2 ng/mL.

Human

E. coli

C-His

Q9NZH7-2 (R5-E157)

27177  [NCBI]

MREAAPKSYAIRDSRQMVWVLSGNSLIAAPLSRSIKPVTLHLIACRDTEFSDKEKGNMVYLGIKGKDLCLFCAEIQGKPTLQLKEKNIMDLYVEKKAQKPFLFFHNKEGSTSVFQSVSYPGWFIATSTTSGQPIFLTKERGITNNTNFYLDSVE

Approximately 18.17 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.