Animal-Free IL-36RN 蛋白, Human (His)

IL-36RN protein tightly regulates immunity by inhibiting the activity of interleukin 36 (IL36A, IL36B, IL36G). It binds to the IL-36 receptor (IL1RL2), preventing binding to IL1RAP and blocking downstream signaling. Animal-Free IL-36RN Protein, Human (His) is the recombinant human-derived animal-FreeIL-36RN protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-36RN Protein, Human (His) is 108 a.a., with molecular weight of ~17.77 kDa.

IL-36RN 蛋白通过抑制白细胞介素 36（IL36A、IL36B 和 IL36G）的活性在免疫调节中发挥关键作用。这种抑制是通过其与 IL-36 受体 (IL1RL2) 的结合来实现的，阻止其与辅助受体 IL1RAP 的结合，从而阻碍下游信号传导。作为 IL-36 信号系统的一部分，类似于 IL-1 系统，IL-36RN 被认为在上皮屏障中发挥作用，有助于局部炎症反应。这种蛋白质与皮肤炎症有关，并被认为参与针对真菌病原体的先天免疫反应，其在对抗烟曲霉方面的潜在作用就是例证。此外，IL-36RN 可能通过招募 SIGIRR 来激活抗炎信号通路。值得注意的是，它与货物受体 TMED10 的相互作用促进从细胞质易位到 ERGIC，从而实现分泌并强调其多方面的调节功能。

Measure by its ability to inhibit IL-36 gamma-induced IL-8 secretion in PBMC cells. The ED₅₀ for this effect is <2 ng/mL in the presence of 500 ng/mL of recombinant human IL-36 gamma.

Human

E. coli

C-His

Q9UBH0 (M1-T108)

26525  [NCBI]

MVLSGALCFRMKDSALKVLYLHNNQLLAGGLHAGKVIKGEEISVVPNRWLDASLSPVILGVQGGSQCLSCGVGQEPTLTLEPVNIMELYLGAKESKSFTFYRRDMGLT

Approximately 17.77 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.