Animal-Free TGF beta 2/TGFB2 蛋白, Human (His)

In the TGF-beta-2 pathway, TGF beta 2/TGFB2 protein acts as a precursor for Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2), regulating their formation. Crucially, TGF beta 2/TGFB2 maintains TGF-beta-2 in a latent state within the extracellular matrix, forming non-covalent associations with TGF-beta-2. Interactions with 'milieu molecules' like LTBP1 and LRRC32/GARP finely tune TGF-beta-2 activation, playing a pivotal role in controlling this critical signaling pathway. Animal-Free TGF beta 2/TGFB2 Protein, Human (His) is the recombinant human-derived animal-FreeTGF beta 2/TGFB2 protein, expressed by E. coli, with C-His labeled tag. The total length of Animal-Free TGF beta 2/TGFB2 Protein, Human (His) is 112 a.a., with molecular weight of ~13.66 kDa.

作为 TGF-β-2 信号通路的核心参与者，TGF-β2/TGFB2 蛋白承担潜伏相关肽 (LAP) 和转化生长因子 β-2 (TGF-β- 2）链条。这些链共同分别形成 TGF-β-2 的调节亚基和活性亚基。值得注意的是，TGF-β2/TGFB2 对于在细胞外基质内储存期间维持 TGF-β-2 链处于潜在状态至关重要。该蛋白与 TGF-β-2 进行非共价结合，通过与“环境分子”（包括 LTBP1 和 LRRC32/GARP）相互作用来复杂地调节其激活。这些相互作用在控制 TGF-β-2 的激活中发挥着关键作用，有助于这一关键信号通路的精细调节。

Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED₅₀ for this effect is <0.2 ng/mL. The specific activity of recombinant human TGF beta 2 is >5 x 10⁶ IU/mg.

Human

E. coli

C-His

P61812 (A303-S414)

7042  [NCBI]

MALDAAYCFRNVQDNCCLRPLYIDFKRDLGWKWIHEPKGYNANFCAGACPYLWSSDTQHSRVLSLYNTINPEASASPCCVSQDLEPLTILYYIGKTPKIEQLSNMIVKSCKCS

Approximately 13.66 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.

<0.01 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.