Animal-Free TNF-alpha/TNFSF2 蛋白, Human (His)

TNF-α/TNFSF2 protein is a macrophage-secreted cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR, inducing tumor cell death and acting as a pyrogen. It is associated with cachexia and stimulates cell proliferation and differentiation. Animal-Free TNF-alpha/TNFSF2 Protein, Human (His) is the recombinant human-derived animal-FreeTNF-alpha/TNFSF2 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free TNF-alpha/TNFSF2 Protein, Human (His) is 157 a.a., with molecular weight of ~18.3 kDa.

TNF-α/TNFSF2 蛋白是一种细胞因子，可与 TNFRSF1A/TNFR1 和 TNFRSF1B/TNFBR 结合，主要由巨噬细胞分泌，能够诱导特定肿瘤细胞系的细胞死亡。作为一种强热原，它通过直接作用或刺激白细胞介素-1 分泌而引起发烧，并与恶病质的诱发有关。此外，在特定条件下，TNF-α可以刺激细胞增殖并诱导细胞分化。值得注意的是，在类风湿性关节炎患者中，它通过 FOXP3 去磷酸化损害调节性 T 细胞 (Treg) 功能，上调蛋白磷酸酶 1 (PP1) 的表达，而蛋白磷酸酶 1 (PP1) 使 FOXP3 的关键“Ser-418”残基去磷酸化，从而使 Treg 细胞功能有缺陷。此外，在 RT4v6 膀胱癌细胞系中，BCG 刺激的中性粒细胞与 DIABLO/SMAC 模拟物联合使用时，TNF-α 是细胞死亡的关键介质。它通过抑制胰岛素诱导的 IRS1 酪氨酸磷酸化和葡萄糖摄取来诱导脂肪细胞中的胰岛素抵抗，从而导致 GKAP42 蛋白降解和 TNF 诱导的胰岛素抵抗。此外，它通过与 IL1B 和 IL6 协同诱导 VEGF 产生，在血管生成中发挥作用，并促进破骨细胞生成，有助于骨吸收。最后，TNF 胞内结构域 (ICD) 形式可诱导树突状细胞产生 IL12，突显其对各种生理过程的不同影响。

The ED₅₀ is <0.1 ng/mL as measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D, corresponding to a specific activity of recombinant human TNF alpha is approximately >1 x 10⁷ IU/mg

Human

E. coli

C-His

P01375 (V77-L233)

7124  [NCBI]

MVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

Approximately 18.3 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.