Animal-Free TNF-alpha/TNFSF2 蛋白, Mouse (His)

TNF-α/TNFSF2 protein is secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR, induces tumor cell death and acts as a pyrogen. It is associated with cachexia, stimulates cell proliferation and differentiation, and induces insulin resistance in adipocytes, leading to TNF-induced insulin resistance. Animal-Free TNF-alpha/TNFSF2 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeTNF-alpha/TNFSF2 protein, expressed by E. coli , with C-His, C-His labeled tag. The total length of Animal-Free TNF-alpha/TNFSF2 Protein, Mouse (His) is 156 a.a., with molecular weight of ~18.20 kDa.

TNF-α/TNFSF2 蛋白是一种与 TNFRSF1A/TNFR1 和 TNFRSF1B/TNFBR 结合的细胞因子。主要由巨噬细胞分泌，可诱导某些肿瘤细胞系的细胞死亡。此外，它是一种强热原，通过直接作用或刺激白细胞介素-1 分泌而引起发烧，并与恶病质的诱发有关。在特定条件下，能刺激细胞增殖，促进细胞分化。在脂肪细胞中，它通过抑制胰岛素诱导的 IRS1 酪氨酸磷酸化和葡萄糖摄取来诱导胰岛素抵抗，同时还会导致 GKAP42 蛋白降解，从而导致 TNF 诱导的胰岛素抵抗。TNF-α/TNFSF2 蛋白通过与 IL1B 和 IL6 协同诱导 VEGF 产生，在血管生成中发挥作用。此外，它促进破骨细胞生成，从而介导骨吸收。最后，TNF-α/TNFSF2 蛋白的 TNF 胞内结构域 (ICD) 形式可刺激树突状细胞中 IL12 的产生。

Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED₅₀ for this effect is <40 pg/mL. The specific activity of recombinant mouse TNF alpha is approximately >2.5x 10⁷ IU/mg

Mouse

E. coli

C-His;C-His

P06804 (L80-L235)

21926  [NCBI]

MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL

Approximately 18.20 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.