Annexin A13/ANXA13 蛋白, Dog (P.pastoris, His)

Annexin A13/ANXA13 protein has a high affinity for folic acid and can bind to reduced folic acid derivatives to promote the transport of 5-methyltetrahydrofolate and folic acid analogs into cells. After endocytosis, the FOLR1 protein undergoes conformational changes when exposed to slightly acidic pH, resulting in a significant reduction in its affinity for folate and subsequent release. Annexin A13/ANXA13 Protein, Dog (P.pastoris, His) is the recombinant dog-derived Annexin A13/ANXA13 protein, expressed by P. pastoris , with N-His labeled tag. The total length of Annexin A13/ANXA13 Protein, Dog (P.pastoris, His) is 315 a.a., with molecular weight of ~37.3 kDa.

FOLR1 蛋白通过与叶酸和还原叶酸衍生物结合，促进 5-甲基四氢叶酸和叶酸类似物输送到细胞内部，从而在细胞过程中发挥至关重要的作用。该受体在中性 pH 条件下对叶酸及其类似物具有高亲和力，在胞吞后暴露于微酸性 pH 条件时会发生构象变化，导致其对叶酸的亲和力显着降低并随后释放。这种动态行为有助于其参与正常胚胎发育和细胞增殖，突出了其在基本生物过程中的重要性。

Dog

P. pastoris

N-His

Q29471 (2G-316H)

403935  [NCBI]

GNRHAKAKSHHGFDVDHDAKKLNKACKGMGTDEAAIIEILSSRTSDERQQIKQKYKATYGKDLEEVFKSDLSGNFEKTALALLDRPSEYDARQLQKAMKGLGTDEAVLIEILCTRTNKEIMAIKEAYQRLFDRSLESDVKADTSGNLKAILVSLLQANRDEGDDVDKDLAGQDAKDLYDAGDGRWGTDELAFNEVLAKRSHKQLRATFQAYQILIDKDIEEAIEAETSGDLQKAYLTLVRCARDQEGYFADRLYKSMKGTGTDEETLIHIIVTRAEVDLQGIKAKFQEKYQKSLSDMVRSDTSGDFQKLLVALLH

Approximately 37.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.