ANO1/Anoctamin-1 蛋白, Human (N-His, C-Myc)

ANO1/Anoctamin-1 protein, a calcium-activated chloride channel (CaCC), plays diverse roles in cellular functions. It serves as a major contributor to basal and stimulated chloride conductance in airway epithelial cells, impacting tracheal cartilage development and contributing to airway mucus expression induced by interleukins IL3 and IL8, as well as by the asthma-associated protein CLCA1. ANO1 is indispensable for normal functioning of the interstitial cells of Cajal (ICCs), crucial for electrical pacemaker activity in gastrointestinal smooth muscles, and is involved in transepithelial anion transport and smooth muscle contraction. It is also required for CFTR activation and membrane expression, modulating endoplasmic reticulum Ca(2+) store release. Additionally, ANO1 participates in calcium-activated chloride secretion in human sweat gland epithelial cells, showing increased basal chloride permeability and decreased Ca(2+)-induced chloride permeability. In sensory neurons, ANO1 acts as a heat sensor and modulates spontaneous firing patterns, contributing to calcium-activated chloride channel activity in taste cells and mediating non-histaminergic Mas-related G-protein coupled receptor (MRGPR)-dependent itching in dorsal root ganglion neurons. In the developing brain, ANO1 is essential for the Ca(2+)-dependent process extension of radial glial cells. ANO1/Anoctamin-1 Protein, Human (N-His, C-Myc) is the recombinant human-derived ANO1/Anoctamin-1 protein, expressed by E. coli , with C-Myc, N-10*His labeled tag. The total length of ANO1/Anoctamin-1 Protein, Human (N-His, C-Myc) is 87 a.a., with molecular weight of ~17.6 kDa.

ANO1/Anoctamin-1 蛋白是一种钙激活氯离子通道 (CaCC)，在细胞功能中发挥多种作用。它是气道上皮细胞中基础氯电导和刺激氯电导的主要贡献者，影响气管软骨发育并促进白细胞介素 IL3 和 IL8 以及哮喘相关蛋白 CLCA1 诱导的气道粘液表达。 ANO1 对于卡哈尔间质细胞 (ICC) 的正常功能是不可或缺的，对于胃肠道平滑肌中的电起搏器活动至关重要，并且参与跨上皮阴离子转运和平滑肌收缩。 CFTR 激活和膜表达、调节内质网 Ca(2+) 储存释放也需要它。此外，ANO1 参与人汗腺上皮细胞中钙激活的氯离子分泌，显示基础氯离子渗透性增加和 Ca(2+) 诱导的氯离子渗透性降低。在感觉神经元中，ANO1 充当热传感器并调节自发放电模式，促进味觉细胞中钙激活的氯离子通道活性，并介导背根神经节中非组胺能 Mas 相关 G 蛋白偶联受体 (MRGPR) 依赖性瘙痒神经元。在发育中的大脑中，ANO1 对于放射状胶质细胞的 Ca(2+) 依赖性过程延伸至关重要。

Human

E. coli

C-Myc;N-10*His

Q5XXA6 (S806-A892)

55107  [NCBI]

SFTSDFIPRLVYLYMYSKNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSENKYDISKDFWAVLAARLA

Approximately 17.6 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.