APLN 蛋白, Human (HEK293, Fc)

APLN protein is an endogenous ligand for the apelin receptor (APLNR), drives receptor internalization, and dissociates more strongly from APLNR than (pyroglu)apelin-13. APLN Protein, Human (HEK293, Fc) is the recombinant human-derived APLN protein, expressed by HEK293 , with C-hFc labeled tag. The total length of APLN Protein, Human (HEK293, Fc) is 55 a.a., with molecular weight of 32-40 kDa.

APLN 蛋白作为 apelin 受体 (APLNR) 的内源配体出现，积极参与受体的内化。值得注意的是，APLN 表现出不同的解离特性，其中 apelin-36 比 (pyroglu)apelin-13 从 APLNR 解离得更牢固。除了作为配体的作用外，APLN 还具有多种生理功能，影响原肠胚形成和心脏形态发生过程中的心脏前体细胞运动。此外，它对 T 细胞受体/CD3 交联反应中的细胞因子产生具有抑制作用，这表明它在调节免疫反应方面具有潜在作用，特别是通过口服初乳和牛奶对新生儿而言。APLN 还有助于早期冠状血管形成并以 ERK1/2 依赖性方式介导心肌收缩力。此外，它可能通过影响加压素释放和饮酒行为在体液稳态的中枢控制中发挥作用。在微生物感染的情况下，APLN 充当 APLNR 的内源配体，与 CD4 一起作为 HIV-1 感染的替代辅助受体，并对 HIV 进入共表达 CD4 和 APLNR 的细胞表现出抑制活性，而 apelin-36 表现出更大的抑制功效比其他合成的 apelin 衍生物。

Immobilized Human APLN, hFc Tag at 0.5 μg/mL (100 μl/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC₅₀ of 22.2 ng/mL determined by ELISA.

Human

HEK293

C-hFc

Q9ULZ1 (G23-F77)

8862  [NCBI]

GSLMPLPDGNGLEDGNVRHLVQPRGSRNGPGPWQGGRRKFRRQRPRLSHKGPMPF

32-40 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution in PBS, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.