APOBEC3A 蛋白, Human (His-SUMO)

APOBEC3A protein is a DNA deaminase that limits the mobility of viruses, foreign DNA, and retrotransposons. APOBEC3A Protein, Human (His-SUMO) is the recombinant human-derived APOBEC3A protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of APOBEC3A Protein, Human (His-SUMO) is 199 a.a., with molecular weight of ~39 kDa.

APOBEC3A 蛋白充当 DNA 脱氨酶，特别是胞苷脱氨酶，对病毒、外源 DNA 和逆转录转座子的移动性发挥限制活性。它证明了对腺相关病毒 (AAV) 和人类 T 细胞白血病病毒 1 型 (HTLV-1) 的抗病毒功效，可能会阻碍 LTR 和非 LTR 反转录转座子的移动性。APOBEC3A 选择性地靶向单链 DNA，具有使外源 DNA 中的甲基胞嘧啶和胞嘧啶脱氨基的能力。此外，它还可以诱导核 DNA 和线粒体 DNA 的体细胞超突变，表明它参与基因组多样性。此外，APOBEC3A 可能通过 DNA 去甲基化的活跃过程促进基因表达的表观遗传调控，突显其在细胞防御机制中的多方面作用以及对表观遗传景观的潜在影响。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His;N-SUMO

P31941 (M1-N199)

100913187  [NCBI]

MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTSVKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSWGCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWDTFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN

Approximately 39 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.