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Apolipoprotein E/APOE 蛋白, Mouse (HEK293, His)

目录号: HY-P7532
COA 产品使用指南

Apolipoprotein E 蛋白, Mouse (HEK293, His)在 HEK293 细胞中表达,N 端带有一个 His 标签。Apolipoprotein E 蛋白 以亚型 (isoform) 依赖的方式调节 PKCη 活性和 occludin 在其 Thr 残基上的磷酸化,从而调节紧密连接 (TJs) 的功能。Apolipoprotein E 还促进胆固醇释放。

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5 μg ¥670 In-stock
10 μg ¥1150 In-stock
50 μg ¥3200 In-stock
100 μg   询价  

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描述

Apolipoprotein E Protein, Mouse (HEK293, His) expresses in HEK293 with a His tag at the N-terminus. Apolipoprotein E regulates tight junction (TJs) function by regulating PKCη activity and phosphorylation of occludin on its Thr residues in an isoform-dependent manner. Apolipoprotein E also promotes cholesterol release[1][2].

研究背景

ApoE 亚型特异性抑制脂质颗粒介导的神经元胆固醇释放。虽然apoE和脂质颗粒是脂质受体,但当apoE和脂质颗粒形成复合物时,颗粒表面的apoE会以apoE浓度依赖性方式抑制脂质颗粒介导的胆固醇从细胞中释放[2]< /sup>。

生物活性

Measured in a cell proliferation assay using SH-SY5Y Human neuroblastoma cells. The ED50 this effect is ≤41.72 ng/ml, corresponding to a specific activity is ≥2.39×10^4 units/mg.

  • Measured in a cell proliferation assay using SH-SY5Y Human neuroblastoma cells. The ED50 for this effect is 41.72 ng/ml, corresponding to a specific activity is 2.39×104 units/mg.
种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

P08226 (E19-Q311)

基因 ID
蛋白结构
N-term
APOE (E19-Q311)
Accession # P08226
6*His
C-term
同用名
rMuApolipoprotein E, His; ApoE; Apolipoprotein E
氨基酸序列

EGEPEVTDQLEWQSNQPWEQALNRFWDYLRWVQTLSDQVQEELQSSQVTQELTALMEDTMTEVKAYKKELEEQLGPVAEETRARLGKEVQAAQARLGADMEDLRNRLGQYRNEVHTMLGQSTEEIRARLSTHLRKMRKRLMRDAEDLQKRLAVYKAGAREGAERGVSAIRERLGPLVEQGRQRTANLGAGAAQPLRDRAQAFGDRIRGRLEEVGNQARDRLEEVREHMEEVRSKMEEQTQQIRLQAEIFQARLKGWFEPIVEDMHRQWANLMEKIQASVATNPIITPVAQENQHHHHHH

分子量

35-40 kDa

纯度
  • Greater than 90% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

Apolipoprotein E/APOE Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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