APP/Protease Nexin-II 蛋白, Human (HEK293, Fc)

There is no specific Pubmed ID mentioned in the paragraph. APP/Protease Nexin-II Protein, Human (HEK293, Fc) is the recombinant human-derived APP/Protease Nexin-II protein, expressed by HEK293 , with C-hFc labeled tag. The total length of APP/Protease Nexin-II Protein, Human (HEK293, Fc) is 652 a.a., with molecular weight of 150-160 kDa.

APP（淀粉样前体蛋白），也称为蛋白酶 Nexin-II，作为一种多功能细胞表面受体，对神经元发挥生理作用，这对神经突生长、神经元粘附和轴突形成至关重要。通过相邻细胞上 APP 分子之间的相互作用促进突触连接，突出了它对突触发生的参与。除了细胞粘附之外，APP 通过蛋白质-蛋白质相互作用在细胞移动性和转录调节中发挥作用。它可以通过与 APBB1-KAT5 结合刺激转录激活，并通过与 Numb 相互作用抑制 Notch 信号传导。此外，APP 与细胞凋亡诱导途径（例如 G(o) 和 JIP 介导的途径）偶联，并抑制 G(o) α ATP 酶活性。APP 作为驱动蛋白 I 膜受体，促进 β-分泌酶和早老素 1 的轴突运输，有助于轴突顺行货物运输至突触。在铜稳态的背景下，APP 参与铜离子还原，并可通过铜金属化相互作用诱导神经元死亡。此外，APP 通过与细胞外基质成分结合来调节神经突生长，并通过其含有 BPTI 结构域的亚型具有蛋白酶抑制剂活性。该蛋白参与 AGER 依赖性途径，激活 p38 MAPK 并诱导淀粉样β肽内化，导致线粒体功能障碍。此外，APP 为 GPC1 提供一氧化氮释放和硫酸乙酰肝素降解所需的 Cu(2+) 离子。它具有金属螯合特性，减少过渡金属，并与脂蛋白、载脂蛋白和高密度脂蛋白颗粒结合，从而调节金属催化的氧化。APP 复杂地参与各种细胞过程，强调了它在正常神经元功能和与神经退行性疾病相关的病理状况中的重要性。

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC₅₀ value is 1.02 nM, as measured with under the described conditions.

Human

HEK293

C-hFc

P05067 (L18-L669)

351  [NCBI]

LEVPTDGNAGLLAEPQIAMFCGRLNMHMNVQNGKWDSDPSGTKTCIDTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHPHFVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDNVDSADAEEDDSDVWWGGADTDYADGSEDKVVEVAEEEEVAEVEEEEADDDEDDEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSAIPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPRHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHSFGADSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFRHDSGYEVHHQKL

150-160 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.