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AQP1 蛋白, Human (His-SUMO)

目录号: HY-P72085
产品使用指南

AQP1 蛋白形成水特异性通道,允许水穿过红细胞和肾近端小管膜。AQP1 蛋白, Human (His-SUMO) 是重组的 AQP1 蛋白,由 E. coli 表达,带有 N-6*His, N-SUMO 标签。AQP1 蛋白, Human (His-SUMO) 全长 50 个氨基酸,分子量约为 ~21.6 kDa。

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描述

The AQP1 protein forms water-specific channels that allow water to cross red blood cells and renal proximal tubule membranes. AQP1 Protein, Human (His-SUMO) is the recombinant human-derived AQP1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of AQP1 Protein, Human (His-SUMO) is 50 a.a., with molecular weight of ~21.6 kDa.

研究背景

AQP1 是一种水特异性通道,在增强红细胞和肾近曲小管质膜的通透性方面发挥着关键作用,允许水沿着渗透梯度移动。它形成同源四聚体,是锚蛋白-1 复合物的关键成分,锚蛋白-1 复合物是一种对维持红细胞膜稳定性和形状至关重要的多蛋白组装体。在红细胞中,ankyrin-1 复合物由 AQP1 以及 ANK1、RHCE、RHAG、SLC4A1、EPB42、GYPA 和 GYPB 组成。AQP1 与 EPHB2 的相互作用进一步凸显了 AQP1 的多功能性,有助于内耳内淋巴的产生。此外,AQP1 参与涉及 STOM 的复合体。该蛋白质通过其 N 端区域与 ANK1 以及通过其 C 端区域与 EPB42 建立相互作用,强调其参与各种细胞过程和蛋白质组装。

种属

Human

表达系统

E. coli

标签

N-6*His;N-SUMO

蛋白编号

P29972 (G220-K269)

基因 ID

358  [NCBI]

蛋白结构
N-term
6*His-SUMO
AQP1 (G220-K269)
Accession # P29972
C-term
同用名
AQP 1; AQP CHIP; AQP-1; AQP1; AQP1_HUMAN; aquaporin 1 channel-forming integral protein; 28kDa; CO blood group; aquaporin 1 Colton blood group; Aquaporin CHIP; Aquaporin-1; Aquaporin-CHIP; Aquaporin1; Channel forming integral protein 28kDa; Channel like integral membrane protein 28 kDa; CHIP 28; CHIP28; CO; Colton blood group; Growth factor induced delayed early response protein; MGC26324; Urine water channel; Water channel protein CHIP 29; Water channel protein CHIP29; Water channel protein for red blood cells and kidney proximal tubule
氨基酸序列

GALAVLIYDFILAPRSSDLTDRVKVWTSGQVEEYDLDADDINSRVEMKPK

分子量

Approximately 21.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

AQP1 Protein, Human (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
AQP1 Protein, Human (His-SUMO)
目录号:
HY-P72085
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