1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Arginase-1/ARG1 蛋白, Human (HEK293, His)

Arginase-1/ARG1 蛋白, Human (HEK293, His)

目录号: HY-P7602
COA 产品使用指南

Arginase-1 蛋白, Human (HEK293, His)在 HEK293 细胞中表达,C 端带有 His 标签。Arginase-1 蛋白 抑制小鼠中抗精氨酸消耗酶精氨酸脱亚氨酶 (ADI) 的 Hep3B 肿瘤细胞的生长。

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规格 价格 是否有货 数量
10 μg ¥2000 In-stock
50 μg ¥6000 In-stock
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250 μg   询价  

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描述

Arginase-1 Protein, Human (HEK293, His) expresses in HEK293 cells with a His tag at the C-terminus. Arginase-1 Protein inhibits the growth of the arginine-depleting enzyme arginine deiminase (ADI)-resistant Hep3B tumor cells in mice[1].

研究背景

Recombinant Human Arginase-1 has IC50s of 0.18 U/mL and 0.07 U/mL for HepG2 and Hep3B cells. Recombinant Human Arginase-1 has a Km value of 1.9 mM for arginine[1].

生物活性

Measured by the production of urea during the hydrolysis of arginine. The specific activity is 4.6 ×104 - 1.5 ×108pmol/min/µg.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P05089 (M1-K322)

基因 ID

383  [NCBI]

同用名
rHuArginase-1, His; ARG1; Arginase-1
氨基酸序列

MSAKSRTIGIIGAPFSKGQPRGGVEEGPTVLRKAGLLEKLKEQECDVKDYGDLPFADIPNDSPFQIVKNPRSVGKASEQLAGKVAEVKKNGRISLVLGGDHSLAIGSISGHARVHPDLGVIWVDAHTDINTPLTTTSGNLHGQPVSFLLKELKGKIPDVPGFSWVTPCISAKDIVYIGLRDVDPGEHYILKTLGIKYFSMTEVDRLGIGKVMEETLSYLLGRKKRPIHLSFDVDGLDPSFTPATGTPVVGGLTYREGLYITEEIYKTGLLSGLDIMEVNPSLGKTPEEVTRTVNTAVAITLACFGLAREGNHKPIDYLNPPKHHHHHH

分子量

Approximately 38.0 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE
性状

Solution

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 20%Glycerol, 1 mM DTT, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献

Arginase-1/ARG1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Arginase-1/ARG1 Protein, Human (HEK293, His)
目录号:
HY-P7602
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