ARMET/MANF 蛋白, Human (158a.a, HEK293, His)

ARMET/MANF Protein, Human (158a.a, HEK293, His) expresses in HEK293 cells with a His tag. Armet/MANF, localized in the ER and Golgi, is a secreted protein and acts as a gene up-regulated by various forms of ER stress^[1].

Armet（也称为 MANF）是一种在不同细胞系中各种形式的 ER 应激以及大鼠脑缺血中上调的基因。Armet 与突变体 matrilin-3 相互作用。Armet 是一种具有底物特异性的基因型特异性 ER 应激反应蛋白，突变体 matrilin-3 的聚集是多发性骨骺发育不良 (MED) 的关键疾病触发因素，可作为潜在的治疗靶点^{[1]< /sup>。}

Human

HEK293

C-6*His

P55145 (L25-L182)

7873  [NCBI]

LRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDLHHHHHH

Approximately 17.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM PB,150 mM NaCl, pH7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Andria Apostolou, et al. Armet, a UPR-upregulated protein, inhibits cell proliferation and ER stress-induced cell death. Exp Cell Res. 2008 Aug 1;314(13):2454-67.  [Content Brief]