ART1 蛋白, Cynomolgus (HEK293, His)

ART1 Protein is an arginine-specific ADP-ribosyltransferase. ART1 is involved in the regulation of a diverse array of pathophysiological processes, including proliferation, invasion, apoptosis, autophagy and angiogenesis of colorectal cancer (CRC) cells. ART1 plays a crucial role in the elevation of glucose consumption in CT26 cells and may regulate GLUT1-dependent glycolysis in CRC via the PI3K/AKT/HIF1α pathway. ART1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived ART1 protein, expressed by HEK293 , with C-His labeled tag. The total length of ART1 Protein, Cynomolgus (HEK293, His) is 272 a.a., with molecular weight of 33-43 KDa.

NAD(P)(+)--精氨酸 ADP-核糖基转移酶 (ART1) 是一种精氨酸特异性 ADP-核糖基转移酶，使用烟酰胺腺嘌呤二核苷酸 (NAD+) 作为底物，催化 ADP-核糖部分转移至受体蛋白上的精氨酸残基，从而引起受体蛋白的活性和功能的变化。ART1 参与多种病理生理过程的调节，包括结直肠癌 (CRC) 细胞的增殖、侵袭、凋亡、自噬和血管生成。ART1 在 CT26 细胞葡萄糖消耗升高中起着至关重要的作用，并可能通过 PI3K/AKT/HIF1α 途径调节 CRC 中 GLUT1 依赖性糖酵解。此外，ART1在高糖条件下参与糖酵解和能量代谢，并通过PI3K/AKT途径促进结直肠癌细胞中的血管生成^[1]。

Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 17.43 ng/mL. Corresponding to a specific activity is 5.737×10^4 Unit/mg.

Cynomolgus

HEK293

C-6*His

XP_005579037 (Q23-N294)

102142531  [NCBI]

QSHPITRRDLFSQEMPLDMALASFDDQYAGCAAAMTAALPDLNHTEFQANKVYADGWTLASSQWQERQAWGPEWSLSPTRPPPPPLGFRDEHGVALLAYTANSPLHKEFNAAVREAGRSRAHYLHHFSFKTLHFLLTEALQLLGRGQRPPRCHQVFRGVHGLHFRPAGPGATVRLGGFASASLKNVAAQQFGEDTFFGIWTCLGAPIKGYSFFPGEEEVLIPPFETFQVINASRPAQGPARIYLRALGKRSTYNCEYIKDKKCKSGPCHLDN

Approximately 33-34 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.