1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Arylsulfatase A/ARSA 蛋白, Human (489a.a, HEK293, C-His)

Arylsulfatase A/ARSA 蛋白, Human (489a.a, HEK293, C-His)

目录号: HY-P7608A
COA 产品使用指南

芳基硫酸酯酶 A (ARSA) 蛋白专门水解硫酸脑苷脂,证明了其在分解这种特定底物中的关键作用。Arylsulfatase A/ARSA 蛋白, Human (489a.a, HEK293, C-His) 是重组的 芳基硫酸酯酶 A/ARSA 蛋白,由 HEK293 表达,带有 C-6*His 标签。Arylsulfatase A/ARSA 蛋白, Human (489a.a, HEK293, C-His) 全长 489 个氨基酸,分子量约为 approximately 65 kDa。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Arylsulfatase A (ARSA) protein specializes in hydrolyzing cerebroside sulfate, demonstrating its key role in breaking down this particular substrate. Arylsulfatase A/ARSA Protein, Human (489a.a, HEK293, C-His) is the recombinant human-derived Arylsulfatase A/ARSA protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Arylsulfatase A/ARSA Protein, Human (489a.a, HEK293, C-His) is 489 a.a., with molecular weight of approximately 65 kDa.

研究背景

芳基硫酸酯酶 A (ARSA) 蛋白专门负责硫酸脑苷脂的水解。这种酶功能涉及硫酸脑苷脂的裂解,展示了 ARSA 在这种特定底物分解中的作用。

生物活性

Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS). The specific activity is 9.75×105 pmol/min/µg, as measured under the described conditions.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P15289 (R19-A507)

基因 ID

410  [NCBI]

蛋白结构
N-term
ARSA (R19-A507)
Accession # P15289
6*His
C-term
同用名
rHuARSA, His; Arylsulfatase A; ARSA
氨基酸序列

RPPNIVLIFADDLGYGDLGCYGHPSSTTPNLDQLAAGGLRFTDFYVPVSLCTPSRAALLTGRLPVRMGMYPGVLVPSSRGGLPLEEVTVAEVLAARGYLTGMAGKWHLGVGPEGAFLPPHQGFHRFLGIPYSHDQGPCQNLTCFPPATPCDGGCDQGLVPIPLLANLSVEAQPPWLPGLEARYMAFAHDLMADAQRQDRPFFLYYASHHTHYPQFSGQSFAERSGRGPFGDSLMELDAAVGTLMTAIGDLGLLEETLVIFTADNGPETMRMSRGGCSGLLRCGKGTTYEGGVREPALAFWPGHIAPGVTHELASSLDLLPTLAALAGAPLPNVTLDGFDLSPLLLGTGKSPRQSLFFYPSYPDEVRGVFAVRTGKYKAHFFTQGSAHSDTTADPACHASSSLTAHEPPLLYDLSKDPGENYNLLGGVAGATPEVLQALKQLQLLKAQLDAAVTFGPSQVARGEDPALQICCHPGCTPRPACCHCPDPHAHHHHHH

分子量

approximately 65 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCL, 150 mM NaCl, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Arylsulfatase A/ARSA Protein, Human (489a.a, HEK293, C-His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Arylsulfatase A/ARSA Protein, Human (489a.a, HEK293, C-His)
目录号:
HY-P7608A
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