Arylsulfatase A/ARSA 蛋白, Mouse (HEK293, His)

Arylsulfatase A (ARSA) possesses arylsulfatase and calcium ion binding activities. It plays a critical role in sperm-zona pellucida binding and is located in the plasma membrane. ARSA is expressed in the brain and genitourinary system, reflecting its involvement in various processes. It is associated with metachromatic leukodystrophy and broadly expressed across tissues, highlighting its significance in diverse contexts. Arylsulfatase A/ARSA Protein, Mouse (HEK293, His) is the recombinant mouse-derived Arylsulfatase A/ARSA protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Arylsulfatase A/ARSA Protein, Mouse (HEK293, His) is 489 a.a., with molecular weight of ~65 kDa.

芳基硫酸酯酶 A (ARSA) 预计具有芳基硫酸酯酶活性和钙离子结合活性。它在精子与透明带结合活性的上游发挥着关键作用，并且位于质膜中，作为膜的组成部分。ARSA 在大脑和泌尿生殖系统中表达，反映其参与各种生理过程。该基因与异染性脑白质营养不良有关，这种疾病的特征是脑硫苷脂在中枢和周围神经系统中异常积累。ARSA 在组织中广泛表达，包括睾丸、肺和许多其他器官，强调了其在不同生物学背景中的重要性。

Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS). The specific activity is 9.387×10^4 pmoL/min/µg, as measured under the described conditions.

Mouse

HEK293

C-10*His

NP_033843.2 (S18-S506)

11883  [NCBI]

SPPNILLIFADDLGYGDLGSYGHPSSTTPNLDQLAEGGLRFTDFYVPVSLCTPSRAALLTGRLPVRSGMYPGVLGPSSQGGLPLEEVTLAEVLAARGYLTGMAGKWHLGVGPEGAFLPPHQGFHRFLGIPYSHDQGPCQNLTCFPPDIPCKGGCDQGLVPIPLLANLTVEAQPPWLPGLEARYVSFSRDLMADAQRQGRPFFLYYASHHTHYPQFSGQSFTKRSGRGPFGDSLMELDGAVGALMTTVGDLGLLEETLVIFTADNGPELMRMSNGGCSGLLRCGKGTTFEGGVREPALVYWPGHITPGVTHELASSLDLLPTLAALTGAPLPNVTLDGVDISPLLLGTGKSPRKSVFFYPPYPDEIHGVFAVRNGKYKAHFFTQGSAHSDTTSDPACHAANRLTAHEPPLLYDLSQDPGENYNVLESIEGVSPEALQALKHIQLLKAQYDAAMTFGPSQIAKGEDPALQICCQPSCTPHPVCCHCPGSQS

Approximately 65 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.