ASGR1/ASGPR1 蛋白, Human (HEK293, His)

ASGR1/ASGPR1 Protein, Human (HEK293, His), a recombinant human Asialoglycoprotein Receptor 1 produced in HEK293 cells, has a His tag. Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells^[1].

长期以来，去唾液酸糖蛋白受体 1 (ASGR1) 一直被认为是肝脏表面标志物，并已用于鉴定循环肝细胞癌细胞、纯化 hPSC 衍生的肝细胞样细胞 (HLC) 以及证明 HLC 与 hPSC 分化的效率。ASGR1 标记了白蛋白阳性 HLC 的一个子集，它们比未纯化的细胞更类似于成熟的肝细胞^[1]。

Human

HEK293

C-6*His

P07306 (Q62-L291)

432  [NCBI]

QNSQLQEELRGLRETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTWMGLHDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDDGRWNDDVCQRPYRWVCETELDKASQEPPLLHHHHHH

Approximately 38.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Derek T Peters, et al. Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells. Development. 2016 May 1;143(9):1475-81.  [Content Brief]