1. 重组蛋白
  2. Receptor Proteins
  3. ASGR2/ASGPR2 蛋白, Mouse (His-SUMO)

ASGR2/ASGPR2 蛋白, Mouse (His-SUMO)

目录号: HY-P72091
产品使用指南

ASGR2/ASGPR2 蛋白在细胞过程中至关重要,介导去唾液酸化血浆糖蛋白的内吞作用。它识别末端半乳糖和 N-乙酰半乳糖胺,促进配体内化,并形成转运至分选细胞器的复合物。ASGR2/ASGPR2 蛋白, Mouse (His-SUMO) 是重组的 ASGR2/ASGPR2 蛋白,由 E. coli 表达,带有 N-10*His, N-SUMO 标签。ASGR2/ASGPR2 蛋白, Mouse (His-SUMO) 全长 222 个氨基酸,分子量约为 ~45.9 kDa。

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描述

ASGR2/ASGPR2 proteins are critical in cellular processes, mediating the endocytosis of desialylated plasma glycoproteins. It recognizes terminal galactose and N-acetylgalactosamine, promotes ligand internalization, and forms complexes that are transported to sorting organelles. ASGR2/ASGPR2 Protein, Mouse (His-SUMO) is the recombinant mouse-derived ASGR2/ASGPR2 protein, expressed by E. coli , with N-10*His, N-SUMO labeled tag. The total length of ASGR2/ASGPR2 Protein, Mouse (His-SUMO) is 222 a.a., with molecular weight of ~45.9 kDa.

研究背景

ASGR2/ASGPR2 蛋白通过介导血浆糖蛋白的内吞作用在细胞过程中发挥至关重要的作用,血浆糖蛋白的复杂碳水化合物部分上的末端唾液酸残基已被去除。该受体识别末端半乳糖和 N-乙酰半乳糖胺单元,促进配体的内化,形成复合物,随后转运至分选细胞器。在该细胞器内,受体和配体解离,然后受体再循环回到细胞膜表面。ASGR2/ASGPR2 参与这些动态过程凸显了其在细胞糖蛋白处理中的重要性,并有助于细胞稳态的调节。该蛋白还与 LASS2 相互作用,进一步扩展其分子关联以及对细胞信号传导或协调的潜在影响。

种属

Mouse

表达系统

E. coli

标签

N-10*His;N-SUMO

蛋白编号

P24721 (Q80-H301)

基因 ID

11890  [NCBI]

蛋白结构
N-term
10*His-SUMO
ASGR2 (Q80-H301)
Accession # P24721
C-term
同用名
Asgr2; Asgr-2; Asialoglycoprotein receptor 2; ASGP-R 2; ASGPR 2; Hepatic lectin 2; HL-2; mHL-2
氨基酸序列

QSIQLQEEFRTLKETFSNFSSSTLMEFGALDTLGGSTNAILTSWLAQLEEKQQQLKADHSTLLFHLKHFPMDLRTLTCQLAYFQSNGTECCPVNWVEFGGSCYWFSRDGLTWAEADQYCQLENAHLLVINSREEQDFVVKHRSQFHIWIGLTDRDGSWKWVDGTDYRSNYRNWAFTQPDNWQGHEQGGGEDCAEILSDGHWNDNFCQQVNRWVCEKRRNITH

分子量

Approximately 45.9 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ASGR2/ASGPR2 Protein, Mouse (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ASGR2/ASGPR2 Protein, Mouse (His-SUMO)
目录号:
HY-P72091
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