ASS1 蛋白, Human (His)

ASS1 Protein, Human (His) is a recombinant human Argininosuccinate synthase expressed in E. coli with a His tag. Argininosuccinate synthase is an enzyme that catalyzes the synthesis of argininosuccinate from citrulline and aspartate^[1].

人体所有组织都需要精氨酸来合成蛋白质，某些组织也需要精氨酸来满足特殊需要。精氨酸的任何“从头”生物合成途径都涉及由精氨基琥珀酸合酶和精氨基琥珀酸裂合酶催化的瓜氨酸向精氨酸的转化。具体而言，精氨基琥珀酸合酶催化瓜氨酸和天冬氨酸缩合形成精氨基琥珀酸，即精氨酸的直接前体^[1]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P00966 (M1-K412)

445  [NCBI]

HHHHHHMSSKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDVSREFVEEFIWPAIQSSALYEDRYLLGTSLARPCIARKQVEIAQREGAKYVSHGATGKGNDQVRFELSCYSLAPQIKVIAPWRMPEFYNRFKGRNDLMEYAKQHGIPIPVTPKNPWSMDENLMHISYEAGILENPKNQAPPGLYTKTQDPAKAPNTPDILEIEFKKGVPVKVTNVKDGTTHQTSLELFMYLNEVAGKHGVGRIDIVENRFIGMKSRGIYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIAKSQERVEGKVQVSVLKGQVYILGRESPLSLYNEELVSMNVQGDYEPTDATGFININSLRLKEYHRLQSKVTAK

Approximately 50.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM PB, 150 mM NaCl, 50 mM Imidazole, 1 mM DTT, 40% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Ricci J Haines, et al. Argininosuccinate synthase: at the center of arginine metabolism. Int J Biochem Mol Biol. 2011;2(1):8-23.  [Content Brief]