1. 重组蛋白
  2. Enzymes & Regulators
  3. Translocases (EC 7)
  4. ATP1B2 蛋白, Human (HEK293, His)

ATP1B2 蛋白, Human (HEK293, His)

目录号: HY-P75587
COA 产品使用指南

ATP1B2 蛋白是 ATP 水解酶的非催化成分,促进跨质膜的 Na(+)/K(+) 离子交换。它的作用对于介导神经元和星形胶质细胞的细胞粘附、促进粘性细胞相互作用至关重要。ATP1B2 蛋白, Human (HEK293, His) 是重组的 ATP1B2 蛋白,由 HEK293 表达,带有 N-His 标签。ATP1B2 蛋白, Human (HEK293, His) 全长 223 个氨基酸,分子量约为 40-60 kDa。

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描述

The ATP1B2 protein is the non-catalytic component of ATP hydrolase and promotes Na(+)/K(+) ion exchange across the plasma membrane. Its role is critical in mediating cell adhesion of neurons and astrocytes, promoting sticky cell interactions. ATP1B2 Protein, Human (HEK293, His) is the recombinant human-derived ATP1B2 protein, expressed by HEK293 , with N-His labeled tag. The total length of ATP1B2 Protein, Human (HEK293, His) is 223 a.a., with molecular weight of 40-60 kDa.

研究背景

ATP1B2 蛋白作为活性酶的非催化成分,负责催化 ATP 水解以及跨质膜的 Na(+) 和 K(+) 离子交换。虽然 beta-2 亚基的精确功能仍然难以捉摸,但它在介导神经元和星形胶质细胞的细胞粘附方面发挥着至关重要的作用,有助于这些细胞之间的粘附相互作用。此外,ATP1B2 促进神经突生长,表明它参与调节神经元投射延伸的复杂过程。这些细胞功能凸显了 ATP1B2 不仅在离子运输方面的重要性,而且在介导粘附和促进神经网络结构发育方面的重要性。

种属

Human

表达系统

HEK293

标签

N-6*His

蛋白编号

P14415 (D68-T290)

基因 ID

482  [NCBI]

蛋白结构
N-term
His
ATP1B2 (D68-T290)
Accession # P14415
C-term
同用名
Sodium/potassium-transporting ATPase subunit beta-2; AMOG; ATP1B2
氨基酸序列

DHTPKYQDRLATPGLMIRPKTENLDVIVNVSDTESWDQHVQKLNKFLEPYNDSIQAQKNDVCRPGRYYEQPDNGVLNYPKRACQFNRTQLGNCSGIGDSTHYGYSTGQPCVFIKMNRVINFYAGANQSMNVTCAGKRDEDAENLGNFVMFPANGNIDLMYFPYYGKKFHVNYTQPLVAVKFLNVTPNVEVNVECRINAANIATDDERDKFAGRVAFKLRINKT

分子量

Approximately 40-60 kDa due to the glycosylation.

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ATP1B2 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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