Azoreductase/NQO1 蛋白, Mouse (P.pastoris, His)

The azoreductase/NQO1 protein reduces quinones to hydroquinone using NADH or NADPH as an electron donor. Azoreductase/NQO1 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived Azoreductase/NQO1 protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of Azoreductase/NQO1 Protein, Mouse (P.pastoris, His) is 273 a.a., with molecular weight of ~33 kDa.

偶氮还原酶/NQO1 蛋白是一种含黄素的醌还原酶，利用 NADH 或 NADPH 作为电子供体，催化醌双电子还原为氢醌，从而在细胞氧化还原调节中发挥关键作用。通过乒乓动力学机制，该酶依次将电子从 NAD(P)H 转移到其黄素辅因子，然后从还原黄素转移到醌，从而绕过半醌和活性氧的形成。该过程是醌解毒的关键组成部分，调节细胞氧化还原状态。偶氮还原酶/NQO1 还有助于还原质膜氧化还原系统成分，例如辅酶 Q 和维生素醌，产生抗氧化剂氢醌形式，并可能充当超氧化物清除剂。此外，该蛋白质表现出多功能性，因为它可以选择性地激活醌及其衍生物，产生具有DNA交联抗肿瘤潜力的氧化还原反应性氢醌。此外，Azoreductase/NQO1 作为核心 20S 蛋白酶体的看门人，以 NADH 依赖性方式与肿瘤抑制因子 TP53 和 TP73 相互作用，以抑制氧化应激期间其不依赖于泛素的降解。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

P. pastoris

N-6*His

Q64669 (A2-K274)

18104  [NCBI]

AARRALIVLAHSEKTSFNYAMKEAAVEALKKRGWEVLESDLYAMNFNPIISRNDITGELKDSKNFQYPSESSLAYKEGRLSPDIVAEHKKLEAADLVIFQFPLQWFGVPAILKGWFERVLVAGFAYTYAAMYDNGPFQNKKTLLSITTGGSGSMYSLQGVHGDMNVILWPIQSGILRFCGFQVLEPQLVYSIGHTPPDARMQILEGWKKRLETVWEETPLYFAPSSLFDLNFQAGFLMKKEVQEEQKKNKFGLSVGHHLGKSIPADNQIKARK

Approximately 33 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCI, 0.5 M NaCI, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.