B18R 蛋白, Vaccinia virus (HEK293, His)

B18R protein functions as a potent antagonist against the antiviral effects of host IFN-alpha/beta and key IFN-inducible proteins, such as OAS1 involved in viral RNA degradation. It acts as a soluble IFN-alpha receptor, effectively impeding the interaction between host IFN-alpha and its receptor. The protein's ability to interact with host IFNA1 underscores its role in modulating the host's immune response against viral infections. B18R Protein, Vaccinia virus (HEK293, His) is the recombinant Virus-derived B18R protein, expressed by HEK293 , with C-6*His labeled tag. The total length of B18R Protein, Vaccinia virus (HEK293, His) is 332 a.a., with molecular weight of 46-65 kDa.

B18R 蛋白可作为有效拮抗剂，对抗宿主 IFN-α/β 和关键 IFN 诱导蛋白（包括参与病毒 RNA 降解的宿主 OAS1）诱导的抗病毒作用。B18R 作为可溶性 IFN-α 受体，抑制宿主 IFN-α 与其受体之间的相互作用。这种对 IFN-α 信号通路的干扰可以抵消宿主针对病毒感染的先天免疫反应。值得注意的是，B18R 通过与宿主 IFNA1 相互作用来实现其抑制作用。B18R 的多方面作用强调了其作为病毒免疫逃避策略的作用，强调了其在调节宿主抗病毒防御方面的重要性。

Measured by its binding ability in a functional ELISA. Immobilized IFN-α1a at 2 μg/mL (100 μL/well) can bind Vaccinia virus B18R. The ED₅₀ for this effect is 9.681 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized IFN-α1a at 2 μg/mL (100 μL/well) can bind Vaccinia virus B18R. The ED₅₀ for this effect is 9.681 ng/mL.

Virus

HEK293

C-6*His

P25213 (H20-E351)

3707577  [NCBI]

HSYAIDIENEITEFFNKMRDTLPAKDSKWLNPACMFGGTMNDIAALGEPFSAKCPPIEDSLLSHRYKDYVVKWERLEKNRRRQVSNKRVKHGDLWIANYTSKFSNRRYLCTVTTKNGDCVQGIVRSHIRKPPSCIPKTYELGTHDKYGIDLYCGILYAKHYNNITWYKDNKEINIDDIKYSQTGKELIIHNPELEDSGRYDCYVHYDDVRIKNDIVVSRCKILTVIPSQDHRFKLILDPKINVTIGEPANITCTAVSTSLLIDDVLIEWENPSGWLIGFDFDVYSVLTSRGGITEATLYFENVTEEYIGNTYKCRGHNYYFEKTLTTTVVLE

46-65 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.