B3GAT1 蛋白, Human (HEK293, His)

B3GAT1 Protein is pivotal in L2/HNK-1 carbohydrate epitope biosynthesis on glycoproteins and glycosaminoglycan biosynthesis. Catalyzing substrates like ASOR and asialo-fetuin, its enzymatic function depends on sphingomyelin presence, with stearoyl-sphingomyelin being the most effective. Notably, it exclusively acts on saturated fatty acids in sphingomyelin, showing no reactivity with unsaturated fatty acids, regardless of acyl group length. B3GAT1 Protein, Human (HEK293, His) is the recombinant human-derived B3GAT1 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of B3GAT1 Protein, Human (HEK293, His) is 310 a.a., with molecular weight of 50-60 kDa.

B3GAT1 蛋白在糖蛋白上 L2/HNK-1 碳水化合物表位的生物合成中发挥关键作用，并参与糖胺聚糖生物合成。参与其催化活性的底物包括脱唾液酸类粘蛋白（ASOR）、脱唾液酸胎球蛋白和脱唾液酸神经细胞粘附分子。有趣的是，该蛋白质的酶功能取决于鞘磷脂的存在，其中硬脂酰基鞘磷脂被证明是最有效的，其次是棕榈酰基鞘磷脂和木素酰基鞘磷脂。值得注意的是，该蛋白质仅对含有饱和脂肪酸的鞘磷脂表现出活性，而与不饱和脂肪酸没有反应性，无论酰基长度如何。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

N-6*His

Q9P2W7 (H25-I334)

27087  [NCBI]

HQSTLAPLLAVHKDEGSDPRRETPPGADPREYCTSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI

50-60 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.