B7-1/CD80 蛋白, Human (HEK293, Fc)

B7-1/CD80 Protein, Human (HEK293, Fc) is a polypeptide chain with the C-termimal human IgG1 Fc fragment produced in HEK293 cells. CD80 is a costimulatory molecule known for its role in T-cell activation and also in regulating normal and malignant B cellsactivity.

CD80 (B7-1) 和 CD86 (B7-2) 由 APC 表达为细胞表面分子，当 T 细胞与其配体 CD28 和 CD152 (CTLA-4) 相互作用时，负责向 T 细胞传递额外或第二信号。B7.1 (CD80) 和 B7.2 (CD86) 这两个属于 Ig 超家族的相关分子的表达对于 APC 激活 T 细胞的能力至关重要^[1]。

2 µg/mL (100 µL/well) of immoblized recombinant human CTLA-4-Fc can bind biotinylated human B7-1/CD80-Fc with a linear range of 1.22-9.77 ng/mL.

Human

HEK293

C-hFc

P33681 (V35-N242)

941  [NCBI]

VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDN

75-80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Vasilevko V, et al. CD80 (B7-1) and CD86 (B7-2) are functionally equivalent in the initiation and maintenance of CD4+ T-cell proliferation after activation with suboptimal doses of PHA. DNA Cell Biol. 2002 Mar;21(3):137-49.  [Content Brief]