B7-1/CD80 蛋白, Rat (HEK293, His)

B7-1/CD80 Protein, Rat (HEK293, His) is not only expressed on APCs, but also is expressed on T cells. B7-1:PD-L1 interaction can inhibit T cell proliferation and cytokine production^[1].

B7-1 和 PD-L1 与 B7-1:CD28 和 B7-1:CTLA-4 的亲和力中间体相互作用。PD-L1:B7-1 接口与 B7-1:CTLA-4 和 PD-L1:PD-1 接口重叠。B7-1:PD-L1 相互作用可抑制 T 细胞增殖和细胞因子产生^[1]。

Rat

HEK293

C-6*His

O35187 (V30-Q248)

25408  [NCBI]

VGLFQISSGIVGQVSKSVREKALLSCDYKFCSEEQSIHRIYWQKHDKMVLSVISGVPEVWPEYKNRTVYDIANNYSFSLLGLILSDRGTYTCVVQRYEGESYVVKHLTTVELSVRADFPTPNITESGNPSADIKRITCFASGGFPKPRLSWLENGRELNGINTTISQDPESELYTISSQLDFNTTYDHFIDCFIEYGDAHVSQNFTWVKPPEDPPDEKQHHHHHH

38-60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Manish J Butte, et al. Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses. Immunity. 2007 Jul;27(1):111-22.  [Content Brief]