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  4. B7-2/CD86
  5. B7-2/CD86 蛋白, Rabbit (HEK293, His)

B7-2/CD86 蛋白, Rabbit (HEK293, His)

目录号: HY-P72766
COA 产品使用指南

B7-2/CD86 蛋白是一种关键受体,通过 CD28CTLA-4 结合提供对 T 淋巴细胞增殖和白细胞介素 2 产生至关重要的共刺激信号。它对早期T细胞激活起决定性作用,影响24小时内的免疫力或无反应性。B7-2/CD86 蛋白, Rabbit (HEK293, His) 是重组的 B7-2/CD86 蛋白,由 HEK293 表达,带有 C-6*His 标签。B7-2/CD86 蛋白, Rabbit (HEK293, His) 全长 224 个氨基酸,分子量为 39-67 kDa。

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规格 价格 是否有货 数量
10 μg ¥1200 In-stock
50 μg ¥2500 In-stock
100 μg   询价  

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描述

The B7-2/CD86 protein is a key receptor that provides costimulatory signals critical for T lymphocyte proliferation and interleukin-2 production through CD28 or CTLA-4 binding. It plays a decisive role in early T cell activation, affecting immunity or anergy within 24 hours. B7-2/CD86 Protein, Rabbit (HEK293, His) is the recombinant Rabbit-derived B7-2/CD86 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of B7-2/CD86 Protein, Rabbit (HEK293, His) is 224 a.a., with molecular weight of 39-67 kDa.

研究背景

B7-2/CD86 蛋白作为 T 淋巴细胞增殖和白细胞介素 2 生成所需的共刺激信号的关键受体发挥作用,通过与 CD28CTLA-4 结合来实现。它在 T 细胞激活和初始 T 细胞共刺激的早期事件中发挥着至关重要的作用,有助于在激活后 24 小时内做出免疫和无反应的决定。此外,B7-2/CD86 参与 B 细胞功能的调节,影响产生的 IgG(1) 水平。CD40 参与后,它通过磷脂酶 C 和蛋白激酶 C 激活来激活 NF-kappa-B 信号通路。该蛋白形成同二聚体并与 MARCH8 相互作用。此外,它与 PHB1 和 PHB2 结合,用 CD40 引发后相互作用增强。

种属

Rabbit

表达系统

HEK293

标签

C-6*His

蛋白编号

P42071 (A23-R246)

基因 ID

100008999  [NCBI]

蛋白结构
N-term
B7-2 (A23-R246)
Accession # P42071
6*His
C-term
同用名
T-lymphocyte activation antigen CD86; Activation B7-2 antigen; CD86  
氨基酸序列

AASLRIQAYFNKTADLPCQFTNSQSRSLSELVVFWQDQERLVLYELFLGREKPDNVDPKYIGRTSFDQESWNLQLHNVQIKDKGVYQCFVHHRGAKGLVPIYQMNSELSVLANFTQPEITLISNITRNSAINLTCSSVQGYPEPKKMFFVLKTENATTEYDGVIEKSQDNVTGLYNISISGSITFSDDIRNATIYCVLQTESTETYSQHFPIVPADPVPVEKPR

分子量

39-67 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
B7-2/CD86 Protein, Rabbit (HEK293, His)
目录号:
HY-P72766
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