1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens
  3. TNF Superfamily Macrophage CD Proteins
  4. TNF Receptor Superfamily BAFF R/CD268
  5. BAFF Receptor
  6. BAFFR/TNFRSF13C 蛋白, Mouse (HEK293, Fc)

BAFFR/TNFRSF13C 蛋白, Mouse (HEK293, Fc)

目录号: HY-P7646
COA 产品使用指南

BAFF Receptor (B-cell activating factor receptor,BAFF-R),又名 TNFRSF13C 和BR3,是识别 BAFF 的 TNF 受体超家族的一种膜蛋白。BAFF Receptor 是 B 细胞存活的关键受体,也是 B 细胞和 T 细胞活化的有效共刺激因子。BAFFR/TNFRSF13C 蛋白, Mouse (HEK293, Fc) 是一种由 HEK293 细胞表达的重组蛋白,其 C 末端带有 His 标签,由 62 个 (S10-A71) 氨基酸组成。

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规格 价格 是否有货 数量
10 μg ¥750 In-stock
50 μg ¥2250 In-stock
100 μg   询价  
250 μg   询价  

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描述

BAFF Receptor (B-cell activating factor receptor, BAFF-R), also known as TNFRSF13C and BR3, is a membrane protein of the TNF receptor superfamily which recognizes BAFF. BAFF Receptor is the crucial receptor for B-cell survival and also is a potent costimulator of both B and T cell activation[1]. BAFFR/TNFRSF13C Protein, Mouse (HEK293, Fc) is a recombinant protein with a C-Terminal Fc label, It consists of 62 amino acids (S10-A71) and is produced in HEK293 cells.

研究背景

BAFF Receptor is expressed on all B cells (except plasma cells), including immature, transitional, mature, memory, and germinal center B cells, as well as on plasma cells[2], while BAFF-R is also expressed on follicular helper T cells (TFH)[3].
The amino acid sequence of human BAFF Receptor protein has low homology for mouse and rat BAFF Receptor protein.
BAFF Receptor binds to BAFF and recruits TNF receptor-associated factor 3 (TRAF-3) and TRAF-2 to the intracellular domain of BAFF-R molecules. The binding of TRAF3 to the BAFF-R reverses the inhibitory effect of unbound/cytoplasmic TRAF3 on the alternative NF-κB2 signaling pathway. It releases NF-κB-inducing kinase (NIK), which phosphorylates inhibitor of κB kinase 1 (IKK1) leading to activation of non-canonical NF-κB. BAFF-R signaling is critical for peripheral B cell survival and differentiation, germinal center formation, plasma cell survival, and IgG and IgE class switching[2].
BAFF Receptor binds to BAFF mediates B-cell survival, proliferation, and differentiation, and involves in the formation of GCs in secondary follicles in murine models and tertiary lymphoid structures in autoimmune diseases[3]. BAFF/BAFF-R signaling is crucial for primary B cell survival, differentiation and homeostasis[4]. A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice[5].

体内研究
(In Vivo)

In BAFF-R-deficient mice, B cells generally develop up to the stage of IgM+ immature/transitional B cells but cannot complete maturation in the spleen[6].

生物活性

Immobilized Human BAFF-His at 10μg/mL (100 μL/well) can bind recombinant Mouse BAFFR/TNFRSF13C, C-Fc (HEK293-expressed). The ED50 of recombinant Mouse BAFFR/TNFRSF13C, C-Fc (HEK293-expressed) is 0.04ug/mL.

种属

Mouse

表达系统

HEK293

标签

C-Fc

蛋白编号

Q9D8D0 (S10-A71)

基因 ID

72049  [NCBI]

中文名
重组小鼠 BAFFR/TNFRSF13C 蛋白 (HEK293, Fc)
同用名
rMuBAFFR/TNFRSF13C, C-Fc; BAFF R; BAFFR; BR3; CD268; TNFRSF13C
氨基酸序列

SQRSRDSSVPTQCNQTECFDPLVRNCVSCELFHTPDTGHTSSLEPGTALQPQEGSALRPDVA

分子量

40-55 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BAFFR/TNFRSF13C Protein, Mouse (HEK293, Fc)
目录号:
HY-P7646
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