1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. BBOX1 蛋白, Human (sf9, His-GST)

BBOX1 蛋白, Human (sf9, His-GST)

目录号: HY-P76163
产品使用指南

BBOX1 蛋白在细胞过程中发挥着核心作用,因为它催化γ-丁基甜菜碱形成左旋肉碱。这种酶活性对于左旋肉碱的生物合成至关重要,左旋肉碱是参与脂肪酸代谢和能量产生的重要化合物。BBOX1 蛋白, Human (sf9, His-GST) 是重组的 BBOX1 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。BBOX1 蛋白, Human (sf9, His-GST) 全长 387 个氨基酸,分子量约为 ~65 KDa。

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描述

The BBOX1 protein plays a central role in cellular processes as it catalyzes the formation of L-carnitine from gamma-butybetaine. This enzyme activity is essential for the biosynthesis of L-carnitine, an important compound involved in fatty acid metabolism and energy production. BBOX1 Protein, Human (sf9, His-GST) is the recombinant human-derived BBOX1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of BBOX1 Protein, Human (sf9, His-GST) is 387 a.a., with molecular weight of ~65 KDa.

研究背景

BBOX1(γ-丁甜菜碱双加氧酶 1)是一种催化 γ-丁甜菜碱形成 L-肉碱的酶。这种酶促过程是左旋肉碱生物合成的关键步骤,左旋肉碱是一种重要化合物,参与将脂肪酸转运到线粒体进行β-氧化。通过将γ-丁甜菜碱转化为左旋肉碱,BBOX1 有助于调节肉碱水平,而肉碱水平在能量代谢中发挥着至关重要的作用。左旋肉碱作为长链脂肪酸跨线粒体膜运输的辅助因子,促进其作为能量来源的利用。参考文献简洁地概述了 BBOX1 在左旋肉碱生物合成中的具体作用,强调了其在细胞代谢和能量稳态中的重要性。

种属

Human

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

O75936 (M1-N387)

基因 ID
蛋白结构
N-term
His-GST
BBOX1 (M1-N387)
Accession # O75936
C-term
同用名
Gamma-butyrobetaine dioxygenase; Gamma-BBH; BBH; BBOX
分子量

Approximately 65 kDa.

纯度

Greater than 85% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

BBOX1 Protein, Human (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BBOX1 Protein, Human (sf9, His-GST)
目录号:
HY-P76163
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