2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. BCAT1 蛋白, Human

BCAT1 蛋白, Human

目录号: HY-P701337
产品使用指南

BCAT1 蛋白在细胞过程中发挥着关键作用，因为它催化必需支链氨基酸亮氨酸、异亮氨酸和缬氨酸分解代谢的初始反应。这种酶活性对于这些氨基酸的分解至关重要，有助于能量产生和氮平衡所需的代谢途径。BCAT1 蛋白, Human 是重组的 BCAT1 蛋白，由 E. coli 表达，不带标签。BCAT1 蛋白, Human 全长 385 个氨基酸。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

The BCAT1 protein plays a key role in cellular processes as it catalyzes the initial reactions in the catabolism of the essential branched-chain amino acids leucine, isoleucine, and valine. This enzyme activity is essential for the breakdown of these amino acids, contributing to metabolic pathways necessary for energy production and nitrogen balance. BCAT1 Protein, Human is the recombinant human-derived BCAT1 protein, expressed by E. coli , with tag free. The total length of BCAT1 Protein, Human is 385 a.a., .
研究背景

BCAT1（支链氨基酸转氨酶 1）是一种催化必需支链氨基酸亮氨酸、异亮氨酸和缬氨酸分解代谢初始反应的酶。这一关键步骤涉及这些氨基酸的可逆转氨作用，将它们转化为各自的支链 α-酮酸。BCAT1 的酶活性对于调节支链氨基酸及其代谢物的水平至关重要，它们在能量代谢和蛋白质合成等各种生理过程中发挥着重要作用。参考文献简洁地概述了 BCAT1 在支链氨基酸分解代谢早期阶段的核心作用，强调其在维持氨基酸稳态和支持重要细胞功能方面的重要性。
种属

Human
表达系统

E. coli
标签

Tag Free
蛋白编号

P54687 (K2-S386)
基因 ID

586
蛋白结构
N-term
BCAT1 (K2-S386)
Accession # P54687
C-term
同用名
BCAT1; Branched-chain-amino-acid aminotransferase; cytosolic; BCAT(c); Protein ECA39
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

BCAT1 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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