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  3. BCCIP 蛋白, Human (sf9)

BCCIP 蛋白, Human (sf9)

目录号: HY-P76746
产品使用指南

BCCIP 蛋白有助于有丝分裂期间的间期微管组织和锚定以及纺锤体极组织和稳定性。BCCIP蛋白可能通过增强CDK2抑制来诱导细胞周期停滞,并通过与BRCA2同源重组参与DNA损伤修复。BCCIP 蛋白, Human (sf9) 是重组的 BCCIP 蛋白,由 Sf9 insect cells 表达,不带标签。BCCIP 蛋白, Human (sf9) 全长 314 个氨基酸,分子量约为 ~47 kDa。

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描述

BCCIP proteins contribute to interphase microtubule organization and anchoring as well as spindle pole organization and stability during mitosis. BCCIP protein may induce cell cycle arrest by enhancing CDK2 inhibition and participate in DNA damage repair through homologous recombination with BRCA2. BCCIP Protein, Human (sf9) is the recombinant human-derived BCCIP protein, expressed by Sf9 insect cells , with tag free. The total length of BCCIP Protein, Human (sf9) is 314 a.a., with molecular weight of ~47 kDa.

研究背景

BCCIP 是一种多面蛋白,在间期和有丝分裂过程中发挥着至关重要的作用。在间期,它对于微管组织和锚定活动至关重要,而在有丝分裂期间,它对纺锤体极的组织和稳定有显着贡献。值得注意的是,与同种型 1/β 相比,同种型 2/α 在调节微管锚定、稳定性、纺锤体结构和方向方面尤其重要。除了其结构功能外,BCCIP 还可以通过增强 CDKN1A 介导的 CDK2 活性抑制来控制细胞周期,从而可能促进细胞周期停滞。此外,BCCIP 可能通过同源重组(可能与 BRCA2 结合)在 DNA 损伤修复中发挥作用,但其在非同源末端连接 (NHEJ) 中的参与尚不清楚。BCCIP 参与多种相互作用,与 BRCA2、CDKN1A、MTDH/LYRIC、DCTN1/p150-glued、ACTR1A/ARP1 和微管蛋白亚基形成复合物。同工型 1 和 2 表现出不同的相互作用特征,两者均与 α-、β- 和 γ-微管蛋白相关。此外,BCCIP 与 TENT5C 相互作用,尽管这种相互作用不会影响 TENT5C 聚 (A) 聚合酶功能。

种属

Human

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

Q9P287 (N-G&P, M1-V314)

基因 ID
蛋白结构
N-term
Gly-Pro
BCCIP (M1-V314)
Accession # Q9P287
C-term
同用名
BRCA2 and CDKN1A-interacting protein; P21- and CDK-associated protein 1; TOK1
分子量

Approximately 47 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

BCCIP Protein, Human (sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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BCCIP Protein, Human (sf9)
目录号:
HY-P76746
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