1. 重组蛋白
  2. Cytokines and Growth Factors CAR-T related Proteins CD Antigens Biotinylated Proteins
  3. TNF Superfamily B Cell CD Proteins
  4. TNF Receptor Superfamily BCMA/CD269
  5. B Cell Maturation Antigen (BCMA)
  6. BCMA/TNFRSF17 Trimer 蛋白, Human (Biotinylated, HEK293, His-Avi)

BCMA/TNFRSF17 Trimer 蛋白, Human (Biotinylated, HEK293, His-Avi)

目录号: HY-P78071
COA 产品使用指南

B Cell Maturation Antigen (BCMA) 也称为 TNFRSF17 或 CD269,是肿瘤坏死因子受体 (TNFR) 超家族的跨膜糖蛋白成员。BCMA 用作多发性骨髓瘤 (MM) 的生物标志物。BCMA 主要在 B 细胞增殖、存活以及分化为浆细胞方面发挥重要作用。BCMA/TNFRSF17 Trimer 蛋白, Human (Biotinylated, HEK293, His-Avi) 是一种由 HEK293 细胞表达的生物素化的重组蛋白,其 C 末端带有一个 Avi 标签和一个 His 标签,由 54 (M1-A54) 个氨基酸组成。

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规格 价格 是否有货 数量
20 μg ¥2450 In-stock
50 μg ¥5400 In-stock
100 μg ¥9180 询价
250 μg   询价  

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描述

B Cell Maturation Antigen (BCMA) also referred to as TNFRSF17 or CD269, is a transmembrane glycoprotein member of the tumor necrosis factor receptor (TNFR) superfamily. BCMA is used as a biomarker for Multiple myeloma (MM). BCMA mainly plays an important role in B cells for their proliferation, survival and also differentiates them into plasma cells[1]. BCMA/TNFRSF17 Trimer Protein, Human (Biotinylated, HEK293, His-Avi) is a biotinylated recombinant protein with a C-Terminal Avi label and a C-Terminal His label, It consists of 54 amino acids (M1-A54) and is produced in HEK293 cells.

研究背景

BCMA is expressed preferentially by mature B lymphocytes, with minimal expression in hematopoietic stem cells or nonhematopoietic tissue[1]. BCMA is almost exclusively expressed on plasmablasts and PCs[2].
The amino acid sequence of human BCMA protein has low homology for mouse BCMA protein.
BCMA is a 184 amino acid and 20.2-kDa type III transmembrane glycoprotein, with the extracellular N terminus containing a conserved motif of 6 cysteines. BCMA has two agonist ligands: a proliferation-inducing ligand (APRIL) and B cell activating factor (BAFF). Upon binding of the ligands to BCMA, activates B cells (NF-κβ), rat sarcoma/mitogen-activated protein kinase (RAS/MAPK), and phosphoinositide-3-kinase-protein kinase B/Akt (PI3K-PKB/Akt) signaling pathway. These pathways result in proliferation stimulation by modulating cell cycle checkpoints, increasing survival by upregulating anti-apoptotic proteins, and production of cell adhesion molecules, angiogenesis factors, and immunosuppressive molecules[2].
BCMA can be used as a promising antigen to target using a variety of immuno-therapy treatments including CART cells, for MM patients[3]. BCMA markedly reduces plasma IgA, IgG, and IgM levels and splenic Ig heavy chain mRNA levels in mouse[4]. In BCMA−/− mice, the long-term survival of PCs is impaired, but lack of BCMA has no effect in short-lived PCs, B cell development, or early humoral immune response, and the splenic architecture and germinal centers appear intact in these BCMA-deficient mice[5]. BCMA overexpression significantly promotes in vivo growth of xenografted MM cells in murine models[6].

体外研究
(In Vitro)

BCMA (human; 10000 ng/mL; 24 h) blocks the activity of ARI2m cells and inhibits the IFNγ production[3].

体内研究
(In Vivo)

BCMA (human; 100 µg; i.p.; on days -4, -2, 0, 2, and 4) decreases the IgA and IgM levels in C57 Bl/6 mice[4].

生物活性

Immobilized Biotinylated Human BCMA (Trimer) at 0.5 μg/mL (100μL/Well) on the plate. Dose response curve for Anti-BCMA Antibody hFc with the EC50 < 8.3 ng/mL determined by ELISA.

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

Q02223 (M1-A54)

基因 ID

608  [NCBI]

中文名
重组人 BCMA/TNFRSF17 蛋白 (Biotinylated, HEK293, His-Avi)
同用名
CD269; TNFRSF17; BCMA; BCM; TNFRSF13A
分子量

35-50 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

Lyophilized powder.

组分

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BCMA/TNFRSF17 Trimer Protein, Human (Biotinylated, HEK293, His-Avi)
目录号:
HY-P78071
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