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BD-1 蛋白, Human

目录号: HY-P7133
COA 产品使用指南

BD-1 蛋白, Human是一种抗菌肽,能够保护上皮表面,包括皮肤,胃肠道和呼吸道。

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规格 价格 是否有货 数量
10 μg ¥1900 In-stock
50 μg ¥4900 In-stock
100 μg   询价  

* Please select Quantity before adding items.

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描述

BD-1 Protein, Human is an antimicrobial peptide that defends epithelial surfaces including the skin, gastrointestinal, and respiratory tracts.

研究背景

重组人 Beta Defensin-1 是一种抗菌肽,可保护上皮表面,包括皮肤、胃肠道和呼吸道[1]。人 Beta Defensin-1 可以为上尿路管腔提供快速作用的抗菌涂层,通过抑制细菌附着到尿路上皮来预防感染,并充当二线化学屏障[2]

生物活性

Full biological activity determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100-1000 ng/mL.

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P60022 (G22-K68)

基因 ID
蛋白结构
N-term
BD-1 (G22-K68)
Accession # P60022
C-term
中文名
重组人 β-防御素-1
同用名
rHuBD-1; HBD1; DEFB1
氨基酸序列

GNFLTGLGHRSDHYNCVSSGGQCLYSACPIFTKIQGTCYRGKAKCCK

分子量

Approximately 5.1 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against 20 mM PBS, pH 7.4, 130 mM NaCl.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

BD-1 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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